Determination of iron(II) and total iron in environmental water samples by flow injection analysis with column preconcentration of chelating resin functionalized with N-hydroxyethylethylenediamine ligands and chemiluminescence detection

A flow injection analysis (FIA) technique for the determination of Fe(II) and total-Fe in environmental water samples has been developed with a high sensitivity. The resin used for preconcentration of iron was the macroporous resin, Amberlite XAD-4 functionalized by N-hydroxyethylethylenediamine (HEED) groups. The technique employed was FIA by combination of on-line chelate resin preconcentration and chemiluminescence detection (CL), using brilliant sulfoflavine and hydrogen peroxide reagent solutions. The interference by coexisting Fe(III) could be eliminated by addition of 1 x 10(-6) mol of deferrioxamine B solution. The detection limits of Fe(II) and total-Fe were 0.80 and 0.36 nmol l(-1) for 5.6-ml seawater samples with a concentration of 2 nmol l(-1). The relative standard deviations for both samples were less than +/- 4%. A typical analysis for Fe(II) can be performed in 7.5 min. The technique was ascertained by comparing the analytical value of total-Fe with the certified value of Fe in the reference standard seawater CASS-3. (C) 1999 Elsevier Science B.V. All rights reserved.