RNAi-Mediated Silencing of EIF3D Alleviates Proliferation and Migration of Glioma U251 and U87MG Cells

被引：23
作者：
Ren, Mingliang [1 ]
Zhou, Chun [1 ]
Liang, Hong [1 ]
Wang, Xuhui [1 ]
Xu, Lunshan [1 ]
机构：
[1] Third Mil Med Univ, Res Inst Field Surg, Daping Hosp, Dept Neurosurg, Chongqing 400042, Peoples R China
来源：
CHEMICAL BIOLOGY & DRUG DESIGN | 2015年 / 86卷 / 04期
基金：
中国国家自然科学基金;
关键词：
EIF3D; glioma; migration; RNA interference; TRANSLATION INITIATION-FACTORS; EIF4E EXPRESSION; EPIDEMIOLOGY; PROTEIN; TEMOZOLOMIDE; COMPLEX; GROWTH; TUMORS;
D O I：
10.1111/cbdd.12542
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
As the most common primary malignant brain tumors, gliomas cause more years of life lost than do any other tumors. Recently, abnormalities of the eukaryotic initiation factors (EIFs) have been reported in gliomas. Yet the role of EIF3D, which encodes a subunit of EIF3 multiprotein complex, remains poorly understood. In this study, we found EIF3D expression was positively correlated with WHO grades of gliomas. Furthermore, we employ lentivirus-mediated RNA interference (RNAi) to examine the physiological role of EIF3D in glioma cells. Decreased EIF3D expression in U251 and U87MG glioma cells caused a delay in cell growth and a disruption in colony formation. In addition, EIF3D knockdown induced G0/G1 phase cell cycle arrest and apoptosis. Cells with suppressed expression of EIF3D had a lower capacity to migrate in the transwell assay. These results suggest that EIF3D plays an important role in glioma development and may serve as a potential therapeutic target for human glioma.
引用
收藏
页码：715 / 722
页数：8
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共 23 条
  • [1] Assessment of Apoptosis by Immunohistochemistry to Active Caspase-3, Active Caspase-7, or Cleaved PARP in Monolayer Cells and Spheroid and Subcutaneous Xenografts of Human Carcinoma
    Bressenot, Aude
    Marchal, Sophie
    Bezdetnaya, Lina
    Garrier, Julie
    Guillemin, Francois
    Plenat, Francois
    [J]. JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY, 2009, 57 (04) : 289 - 300
  • [2] The type II transforming growth factor (TGF)-β receptor-interacting protein TRIP-1 acts as a modulator of the TGF-β response
    Choy, L
    Derynck, R
    [J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 1998, 273 (47) : 31455 - 31462
  • [3] Adult glioblastoma multiforme survival in the temozolomide era: A population-based analysis of Surveillance, Epidemiology, and End Results registries
    Darefsky, Amy S.
    King, Joseph T., Jr.
    Dubrow, Robert
    [J]. CANCER, 2012, 118 (08) : 2163 - 2172
  • [4] eIF4E expression in tumors: its possible role in progression of malignancies
    De Benedetti, A
    Harris, AL
    [J]. INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY, 1999, 31 (01) : 59 - 72
  • [5] Protein kinase D1 regulates matrix metalloproteinase expression and inhibits breast cancer cell invasion
    Eiseler, Tim
    Doeppler, Heike
    Yan, Irene K.
    Goodison, Steve
    Storz, Peter
    [J]. BREAST CANCER RESEARCH, 2009, 11 (01)
  • [6] Therapeutic suppression of translation initiation factor eIF4E expression reduces tumor growth without toxicity
    Graff, Jeremy R.
    Konicek, Bruce W.
    Vincent, Thomas M.
    Lynch, Rebecca L.
    Monteith, David
    Weir, Spring N.
    Schwier, Phil
    Capen, Andrew
    Goode, Robin L.
    Dowless, Michele S.
    Chen, Yuefeng
    Zhang, Hong
    Sissons, Sean
    Cox, Karen
    McNulty, Ann M.
    Parsons, Stephen H.
    Wang, Tao
    Sams, Lillian
    Geeganage, Sandaruwan
    Douglass, Larry E.
    Neubauer, Blake Lee
    Dean, Nicholas M.
    Blanchard, Kerry
    Shou, Jianyong
    Stancato, Louis F.
    Carter, Julia H.
    Marcusson, Eric G.
    [J]. JOURNAL OF CLINICAL INVESTIGATION, 2007, 117 (09) : 2638 - 2648
  • [7] mTOR-dependent stimulation of the association of eIF4G and eIF3 by insulin
    Harris, Thurl E.
    Chi, An
    Shabanowitz, Jeffrey
    Hunt, Donald F.
    Rhoads, Robert E.
    Lawrence, John C., Jr.
    [J]. EMBO JOURNAL, 2006, 25 (08) : 1659 - 1668
  • [8] Viral stress-inducible protein p56 inhibits translation by blocking the interaction of eIF3 with the ternary complex eIF2•GTP•Met-tRNAi
    Hui, DJ
    Bhasker, CR
    Merrick, WC
    Sen, GC
    [J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 2003, 278 (41) : 39477 - 39482
  • [9] The initiation factor eIF3-f is a major target for Atrogin1/MAFbx function in skeletal muscle atrophy
    Lagirand-Cantaloube, Julie
    Offner, Nicolas
    Csibi, Alfredo
    Leibovitch, Marie P.
    Batonnet-Pichon, Sabrina
    Tintignac, Lionel A.
    Segura, Carlos T.
    Leibovitch, Serge A.
    [J]. EMBO JOURNAL, 2008, 27 (08) : 1266 - 1276
  • [10] Knockdown of EIF3D suppresses proliferation of human melanoma cells through G2/M phase arrest
    Li, Hui
    Zhou, Fusheng
    Wang, Hongyan
    Lin, Da
    Chen, Gang
    Zuo, Xianbo
    Sun, Liangdan
    Zhang, Xuejun
    Yang, Sen
    [J]. BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY, 2015, 62 (05) : 615 - 620
123