In Vivo Labeling by CD73 Marks Multipotent Stromal Cells and Highlights Endothelial Heterogeneity in the Bone Marrow Niche

被引：52

作者：

Breitbach, Martin ^{[1
]}

Kimura, Kenichi ^{[1
,2
,3
]}

Luis, Tiago C. ^{[4
]}

Fuegemann, Christopher J. ^{[1
]}

Woll, Petter S. ^{[4
,7
]}

Hesse, Michael ^{[1
,2
]}

Facchini, Raffaella ^{[4
]}

Rieck, Sarah ^{[1
]}

Jobin, Katarzyna ^{[6
]}

Reinhardt, Julia ^{[5
]}

Ohneda, Osamu ^{[3
]}

Wenzel, Daniela ^{[1
]}

Geisen, Caroline ^{[1
,2
]}

Kurts, Christian ^{[6
]}

Kastenmueller, Wolfgang ^{[6
]}

Hoelzel, Michael ^{[5
]}

Jacobsen, Sten E. W. ^{[4
,7
,8
,9
]}

Fleischmann, Bernd K. ^{[1
]}

机构：

[1] Univ Bonn, Inst Physiol 1, Med Fac, Life & Brain Ctr, D-53105 Bonn, Germany

[2] Univ Bonn, Med Fac, Dept Cardiac Surg, D-53105 Bonn, Germany

[3] Univ Tsukuba, Dept Regenerat Med & Stem Cell Biol, Tsukuba, Ibaraki 3058575, Japan

[4] Univ Oxford, Weatherall Inst Mol Med, John Radcliffe Hosp, MRC,Mol Hematol Unit, Oxford OX3 9DS, England

[5] Univ Bonn, Dept Clin Chem & Clin Pharmacol, D-53105 Bonn, Germany

[6] Univ Bonn, Inst Expt Immunol, D-53105 Bonn, Germany

[7] Karolinska Inst, Dept Med Huddinge, Ctr Hematol & Regenerat Med, Stockholm, Sweden

[8] Karolinska Inst, Wallenberg Inst Regenerat Med, Dept Cell & Mol Biol, Stockholm, Sweden

[9] Karolinska Univ Hosp, Stockholm, Sweden

来源：

CELL STEM CELL | 2018年 / 22卷 / 02期

基金：

瑞典研究理事会;

关键词：

MESENCHYMAL STEM-CELLS; HEMATOPOIETIC STEM; PROGENITOR CELLS; GENE-EXPRESSION; RNA-SEQ; CULTURE; MICROENVIRONMENT; TISSUES; SPLEEN; ORGANS;

D O I：

10.1016/j.stem.2018.01.008

中图分类号：

Q813 [细胞工程];

学科分类号：

摘要：

Despite much work studying ex vivomultipotent stromal cells ( MSCs), the identity and characteristics of MSCs in vivo are not well defined. Here, we generated a CD73-EGFP reporter mouse to address these questions and found EGFP(+) MSCs in various organs. In vivo, EGFP(+) mesenchymal cells were observed in fetal and adult bones at proliferative ossification sites, while in solid organs EGFP(+) cells exhibited a perivascular distribution pattern. EGFP(+) cells from the bone compartment could be clonally expanded ex vivo from single cells and displayed trilineage differentiation potential. Moreover, in the central bone marrow CD73-EGFP(+) specifically labeled sinusoidal endothelial cells, thought to be a critical component of the hematopoietic stem cell niche. Purification and molecular characterization of this CD73-EGFP(+) population revealed an endothelial subtype that also displays a mesenchymal signature, highlighting endothelial cell heterogeneity in the marrow. Thus, the CD73-EGFP mouse is a powerful tool for studying MSCs and sinusoidal endothelium.

引用

页码：262 / +

页数：22

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