Differentially expressed long noncoding RNAs and mRNAs in PC12 cells under lysophosphatidylcholine stimulation

被引:4
作者
Zhang, Wen [1 ]
Dun, Su [2 ]
Ping, Yin [3 ]
Wang, Qingliang [4 ]
Tana, Siqin [5 ]
Tana, Aodong [5 ]
Qin, Si [5 ]
Bao, Xilinqiqige [4 ]
Qimuge, Alateng [5 ]
Baiyin, Tegexi [3 ]
Yang, Dezhi [5 ]
Bao, Siqin [5 ]
Baoyin, Seyin [5 ]
Qimuge, Wuhan [5 ,6 ]
机构
[1] Inner Mongolia Univ, Sch Life Sci, Hohhot 010021, Peoples R China
[2] HUA Cloud Intelligent Healthcare Co Ltd, Res & Dev Ctr, Shenzhen 518000, Peoples R China
[3] Inner Mongolia Univ Nationalities, Affiliated Hosp, Tongliao 028000, Peoples R China
[4] Inner Mongolia Med Univ, Hohhot 010010, Peoples R China
[5] Inner Mongolia Int Mongolian Hosp, Hohhot 010065, Peoples R China
[6] Inner Mongolia Tradit Chinese & Mongolian Med Res, Hohhot 010017, Peoples R China
关键词
NEURODEGENERATIVE DISEASES; KINASE; PATHWAY; PROTEIN; ANNOTATION; MECHANISMS; SECRETION; RECEPTOR; KEGG;
D O I
10.1038/s41598-022-21676-5
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Lysophosphatidylcholine (LPC) was previously found to show neuroprotective effect on nerve growth factor (NGF) and brain derived neurotrophic factor (BDNF) induced signalings. Also, numerous studies reported the emerging roles of long noncoding RNAs (LncRNAs) involved in neurodegenerative disease. However, the biological mechanism of LPC and expression profile of lncRNAs has not been reported. Here, lncRNAs in PC12 cells under LPC and NGF treatment were analyzed using high throughput sequencing technology for the first time. We identified 564 annotated and 1077 novel lncRNAs in PC12 cells. Among them, 121 lncRNAs were differentially expressed in the PC12 cells under LPC stimulation. KEGG analysis showed that differentially expressed mRNAs co-expressed with lncRNAs mainly enriched in ribosome, oxidative phosphorylation, Parkinson's disease, Huntington's disease and Alzheimer's disease etc. LncRNA-mRNA network analysis showed that lncRNA ENSRNOT00000082515 had interactions with 626 different mRNAs suggesting that lncRNA ENSRNOT00000082515 probably play vital role. Finally, sequencing data were validated by qRT-PCR for ENSRNOT00000084874, ENSRNOT00000082515, LNC_001033 forward Fgf18, Vcam1, and Pck2.
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页数:12
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