Multimodal evaluation of in vivo magnetic resonance imaging of myocardial restoration by mouse embryonic stem cells

被引：21

作者：

Hendry, Stephen L., II ^{[1
]}

van der Bogt, Koen E. A. ^{[1
,5
]}

Sheikh, Ahmad Y. ^{[1
]}

Arai, Takayasu ^{[2
]}

Dylla, Scott J. ^{[3
]}

Drukker, Micha ^{[3
]}

McConnell, Michael V. ^{[2
]}

Kutschka, Ingo ^{[1
]}

Hoyt, Grant ^{[1
]}

Cao, Feng ^{[4
]}

Weissman, Irving L. ^{[3
]}

Connolly, Andrew J. ^{[3
]}

Pelletier, Marc P. ^{[1
]}

Wu, Joseph C. ^{[2
,4
]}

Robbins, Robert C. ^{[1
]}

Yang, Phillip C. ^{[2
]}

机构：

[1] Stanford Univ, Sch Med, Dept Cardiothorac Surg, Stanford, CA 94305 USA

[2] Stanford Univ, Sch Med, Div Cardiovasc Med, Stanford, CA 94305 USA

[3] Stanford Univ, Sch Med, Dept Pathol, Stanford, CA 94305 USA

[4] Stanford Univ, MIPS, Stanford, CA 94305 USA

[5] Leiden Univ, Med Ctr, Dept Surg, Leiden, Netherlands

来源：

JOURNAL OF THORACIC AND CARDIOVASCULAR SURGERY | 2008年 / 136卷 / 04期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1016/j.jtcvs.2007.12.053

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Objective: Mouse embryonic stem cells have demonstrated potential to restore infarcted myocardium after acute myocardial infarction. Although the underlying mechanism remains controversial, magnetic resonance imaging has provided reliable in vivo assessment of functional recovery after cellular transplants. Multimodal comparison of the restorative effects of mouse embryonic stem cells and mouse embryonic fibroblasts was performed to validate magnetic resonance imaging data and provide mechanistic insight. Methods: SCID-beige mice(n = 55) underwent coronary artery ligation followed by injection of 2.5 x 10(5) mouse embryonic stem cells, 2.5 x 10(5) mouse embryonic fibroblasts, or normal saline solution. In vivo magnetic resonance imaging of myocardial restoration by mouse embryonic stem cells was evaluated by (1) in vivo pressure volume loops, (2) in vivo bioluminescence imaging, and (3) ex vivo TaqMan (Roche Molecular Diagnostics, Pleasanton, Calif) polymerase chain reaction and immunohistologic examination. Results: In vivo magnetic resonance imaging demonstrated significant improvement in left ventricular ejection fraction at 1 week in the mouse embryonic stem cell group. This finding was validated with (1) pressure-volume loop analysis demonstrating significantly improved systolic and diastolic functions, (2) bioluminescence imaging and polymerase chain reaction showing superior posttransplant survival of mouse embryonic stem cells, (3) immunohistologic identification of cardiac phenotype within engrafted mouse embryonic stem cells, and (4) polymerase chain reaction measuring increased expressions of angiogenic and antiapoptotic genes and decreased expressions of antifibrotic genes. Conclusion: This study validates in vivo magnetic resonance imaging as an effective means of evaluating the restorative potential of mouse embryonic stem cells.

引用

页码：1028 / U14

页数：11

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