Presence of functional receptors for corticotropin releasing hormone in caecal circular smooth muscle cells of guinea pig

被引：15

作者：

Iwakiri, Y ^{[1
]}

Chijiiwa, Y ^{[1
]}

Motomura, Y ^{[1
]}

Osame, M ^{[1
]}

Nawata, H ^{[1
]}

机构：

[1] KAGOSHIMA UNIV,FAC MED,DEPT INTERNAL MED 3,KAGOSHIMA 890,JAPAN

来源：

LIFE SCIENCES | 1997年 / 60卷 / 11期

关键词：

corticotropin releasing hormone; specific binding site; isolated smooth muscle cells; caecum;

D O I：

10.1016/S0024-3205(97)00014-3

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

The presence of specific binding sites for corticotropin releasing hormone (CRH) in caecal circular smooth muscle cells of guinea pig was investigated by binding and pharmacological studies. The specific binding of I-125-CRH to these muscle cells reached an equilibrium after 90 minutes. Several peptides structurally unrelated to CRH did not affect the specific binding of I-125-CRH to these muscle cells. Unlabeled CRH completely inhibited the specific binding of I-125-CRH in a concentration-dependent manner, with an IC50 value of 13.5 nM. A CRH receptor antagonist, alpha-helical CRH (9-41), inhibited the specific binding of I-125-CRH in a concentration-dependent manner with a lower affinity than CRH. In pharmacological study, CRH inhibited the contractile response of these muscle cells to 1 nM cholecystokinin-octapeptide in a concentration-dependent manner, with an IC50 value of 14.1 nM. A CRH receptor antagonist, alpha-helical CRH (9-41), significantly antagonized this inhibitory effect produced by CRH. These results strongly suggest the presence of functional receptors for CRH that mediate relaxation of caecal circular smooth muscle cells of guinea pig.

引用

页码：857 / 864

页数：8

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