In Situ Single Cell Mechanics Characterization of Yeast Cells Using Nanoneedles Inside Environmental SEM

被引：54
作者：
Ahmad, Mohd Ridzuan [1 ]
Nakajima, Masahiro [1 ]
Kojima, Seiji [2 ]
Homma, Michio [2 ]
Fukuda, Toshio [1 ]
机构：
[1] Nagoya Univ, Dept Micronano Syst Engn, Chikusa Ku, Nagoya, Aichi 4648603, Japan
[2] Nagoya Univ, Div Biol Sci, Grad Sch Sci, Chikusa Ku, Nagoya, Aichi 4648602, Japan
来源：
IEEE TRANSACTIONS ON NANOTECHNOLOGY | 2008年 / 7卷 / 05期
关键词：
Environmental SEM (ESEM); nanomanipulation; nanoneedles; single cell analysis;
D O I：
10.1109/TNANO.2008.2003185
中图分类号：
TM [电工技术]; TN [电子技术、通信技术];
学科分类号：
0808 ; 0809 ;
摘要：
In this study, characterization of cellular mechanics of W303 yeast cells was conducted using nanoneedles inside an Environmental SEM (ESEM). This enhanced ESEM system comprises a standard ESEM instrument as a nanoimaging tool, a cooling stage as a humidity controller for cellular biology and 7 degrees of freedom and linear actuators as nanomanipulator/effector. Four types of nanoneedles were used in our experiments, i.e., silicon (Si), titanium (Ti) coated Si, and two types of tungsten (W) nanoneedles. The Si and Ti nanoneedles were fabricated using 2 N/m spring constant cantilevers. While the W nanoneedles were fabricated using 0.09 and 2 N/m spring constant cantilevers (W-0.09 and W-2 nanoneedles). The Si, silicon-titanium (Si-Ti), W-0.09, and W-2 nanoneedles are suitable to be used for local stiffness characterization of single cells. This capability can be used in future for fast disease detection since disease cells may show different cell. mechanics properties as compared to the normal cells. The Si-Ti and W-2 nanoneedles can penetrate the cell without cell bursting, and this could be important in single cell surgery in future to avoid cell damage.
引用
收藏
页码：607 / 616
页数：10
相关论文
共 36 条
  • [1] Ahmad MR, 2007, 2007 7TH IEEE CONFERENCE ON NANOTECHNOLOGY, VOL 1-3, P1026
  • [2] Calibration of atomic force microscope cantilevers using piezolevers
    Aksu, Saltuk B.
    Turner, Joseph A.
    [J]. REVIEW OF SCIENTIFIC INSTRUMENTS, 2007, 78 (04)
  • [3] Measurement of local viscoelasticity and forces in living cells by magnetic tweezers
    Bausch, AR
    Möller, W
    Sackmann, E
    [J]. BIOPHYSICAL JOURNAL, 1999, 76 (01) : 573 - 579
  • [4] Applications of carbon nanotubes in drug delivery
    Bianco, A
    Kostarelos, K
    Prato, M
    [J]. CURRENT OPINION IN CHEMICAL BIOLOGY, 2005, 9 (06) : 674 - 679
  • [5] Nanoparticles in cancer therapy and diagnosis
    Brigger, I
    Dubernet, C
    Couvreur, P
    [J]. ADVANCED DRUG DELIVERY REVIEWS, 2002, 54 (05) : 631 - 651
  • [6] The malaria-infected red blood cell: Structural and functional changes
    Cooke, BM
    Mohandas, N
    Coppell, RL
    [J]. ADVANCES IN PARASITOLOGY, VOL 50, 2001, 50 : 1 - 86
  • [7] Computational modeling of the forward and reverse problems in instrumented sharp indentation
    Dao, M
    Chollacoop, N
    Van Vliet, KJ
    Venkatesh, TA
    Suresh, S
    [J]. ACTA MATERIALIA, 2001, 49 (19) : 3899 - 3918
  • [8] The use of environmental scanning electron microscopy for imaging wet and insulating materials
    Donald, AM
    [J]. NATURE MATERIALS, 2003, 2 (08) : 511 - 516
  • [9] Molecules and mechanisms of mechanotransduction
    Goodman, MB
    Lumpkin, EA
    Ricci, A
    Tracey, WD
    Kernan, M
    Nicolson, T
    [J]. JOURNAL OF NEUROSCIENCE, 2004, 24 (42) : 9220 - 9222
  • [10] The optical stretcher:: A novel laser tool to micromanipulate cells
    Guck, J
    Ananthakrishnan, R
    Mahmood, H
    Moon, TJ
    Cunningham, CC
    Käs, J
    [J]. BIOPHYSICAL JOURNAL, 2001, 81 (02) : 767 - 784
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