Allelic diversity in the transcriptomes of contrasting rust-infected genotypes of Lathyrus sativus, a lasting resource for smart breeding

被引:29
作者
Almeida, Nuno Felipe [1 ]
Leitao, Susana Trindade [1 ]
Krezdorn, Nicolas [2 ]
Rotter, Bjoern [2 ]
Winter, Peter [2 ]
Rubiales, Diego [3 ]
Vaz Patto, Maria Carlota [1 ]
机构
[1] Univ Nova Lisboa, Inst Tecnol Quim & Biol Antonio Xavier, Ave Republ, P-2780157 Oeiras, Portugal
[2] GenXPro GmbH, D-60438 Frankfurt, Germany
[3] CSIC, Inst Sustainable Agr, E-14080 Cordoba, Spain
关键词
Grass pea; Partial resistance; Pathogen effectors; RNA-seq; SNP; Uromyces;
D O I
10.1186/s12870-014-0376-2
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Background: Grass pea (Lathyrus sativus L.) is a valuable resource for potentially durable partial resistance to rust. To gain insight into the resistance mechanism and identify potential resistance genes, we generated the first comprehensive transcriptome assemblies from control and Uromyces pisi inoculated leafs of a susceptible and a partially rust-resistant grass pea genotype by RNA-seq. Results: 134,914 contigs, shared by both libraries, were used to analyse their differential expression in response to rust infection. Functional annotation grouped 60.4% of the contigs present in plant databases (37.8% of total) to 33 main functional categories, being "protein", "RNA", "signalling", "transport" and "stress" the most represented. Transcription profiles revealed considerable differences in regulation of major phytohormone signalling pathways: whereas Salicylic and Abscisic Acid pathways were up-regulated in the resistant genotype, Jasmonate and Ethylene pathways were down-regulated in the susceptible one. As potential Resistance-genes we identified a mildew resistance locus O (MLO)-like gene, and MLO-related transcripts. Also, several pathogenesis-related genes were up-regulated in the resistant and exclusively down regulated in the susceptible genotype. Pathogen effectors identified in both inoculated libraries, as e.g. the rust Rtp1 transcript, may be responsible for the down-regulation of defence-related transcripts. The two genotypes contained 4,892 polymorphic contigs with SNPs unevenly distributed between different functional categories. Protein degradation (29.7%) and signalling receptor kinases (8.2%) were the most diverged, illustrating evolutionary adaptation of grass pea to the host/pathogens arms race. Conclusions: The vast array of novel, resistance-related genomic information we present here provides a highly valuable resource for future smart breeding approaches in this hitherto under-researched, valuable legume crop.
引用
收藏
页数:15
相关论文
共 92 条
[1]   Transferability of molecular markers from major legumes to Lathyrus spp. for their application in mapping and diversity studies [J].
Almeida, Nuno Felipe ;
Leitao, Susana Trindade ;
Caminero, Constantino ;
Maria Torres, Ana ;
Rubiales, Diego ;
Vaz Patto, Maria Carlota .
MOLECULAR BIOLOGY REPORTS, 2014, 41 (01) :269-283
[2]  
[Anonymous], 1981, HDB LEGUMES WORLD EC
[3]   Role of plant hormones in plant defence responses [J].
Bari, Rajendra ;
Jones, Jonathan D. G. .
PLANT MOLECULAR BIOLOGY, 2009, 69 (04) :473-488
[4]   Mapping of quantitative trait loci controlling partial resistance against rust incited by Uromyces pisi (Pers.) Wint. in a Pisum fulvum L. intraspecific cross [J].
Barilli, E. ;
Satovic, Z. ;
Rubiales, D. ;
Torres, A. M. .
EUPHYTICA, 2010, 175 (02) :151-159
[5]   Clarification on rust species potentially infecting pea (Pisum sativum L.) crop and host range of Uromyces pisi (Pers.) Wint [J].
Barilli, Eleonora ;
Moral, Ana ;
Sillero, Josefina C. ;
Rubiales, Diego .
CROP PROTECTION, 2012, 37 :65-70
[6]   Phylogenetic Analysis of Uromyces Species Infecting Grain and Forage Legumes by Sequence analysis of Nuclear Ribosomal Internal Transcribed Spacer Region [J].
Barilli, Eleonora ;
Satovic, Zlatko ;
Sillero, Josefina C. ;
Rubiales, Diego ;
Torres, Ana M. .
JOURNAL OF PHYTOPATHOLOGY, 2011, 159 (03) :137-145
[7]   Induction of Systemic Acquired Resistance in Pea against Rust (Uromyces pisi) by Exogenous Application of Biotic and Abiotic Inducers [J].
Barilli, Eleonora ;
Sillero, Josefina C. ;
Rubiales, Diego .
JOURNAL OF PHYTOPATHOLOGY, 2010, 158 (01) :30-34
[8]  
Bennett M., 2012, Angiosperm DNA C-values database (release 8.0
[9]  
Campbell C G., 1997, Promoting the conservation and use of underutilized and neglected crops
[10]   The roles of ABA in plant-pathogen interactions [J].
Cao, Feng Yi ;
Yoshioka, Keiko ;
Desveaux, Darrell .
JOURNAL OF PLANT RESEARCH, 2011, 124 (04) :489-499