ER-localized auxin transporter PIN8 regulates auxin homeostasis and male gametophyte development in Arabidopsis

被引：220

作者：

Ding, Zhaojun ^{[1
,2
,3
]}

Wang, Bangjun ^{[4
]}

Moreno, Ignacio ^{[5
]}

Duplakova, Nikoleta ^{[6
]}

Simon, Sibu ^{[1
,2
]}

Carraro, Nicola ^{[7
]}

Reemmer, Jesica ^{[7
]}

Pencik, Ales ^{[8
]}

Chen, Xu ^{[1
,2
]}

Tejos, Ricardo ^{[1
,2
]}

Skupa, Petr ^{[6
]}

Pollmann, Stephan ^{[9
]}

Mravec, Jozef ^{[1
,2
]}

Petrasek, Jan ^{[6
]}

Zazimalova, Eva ^{[6
]}

Honys, David ^{[6
]}

Rolcik, Jakub ^{[8
]}

Murphy, Angus ^{[7
]}

Orellana, Ariel ^{[5
]}

Geisler, Markus ^{[4
]}

Friml, Jiri ^{[1
,2
,10
,11
]}

机构：

[1] Univ Ghent VIB, Dept Plant Syst Biol, B-9052 Ghent, Belgium

[2] Univ Ghent, Dept Plant Biotechnol & Genet, B-9052 Ghent, Belgium

[3] Shandong Univ, Key Lab Plant Cell Engn & Germplasm Innovat, Minist Educ, Sch Life Sci, Jinan 250100, Peoples R China

[4] Univ Fribourg, Dept Biol, CH-1700 Fribourg, Switzerland

[5] Univ Andres Bello, Ctr Genome Regulat, Ctr Plant Biotechnol, Fac Ciencias Biol, Santiago, Chile

[6] Inst Expt Bot ASCR, Prague 16500 6, Czech Republic

[7] Purdue Univ, Dept Hort, W Lafayette, IN 47907 USA

[8] Palacky Univ, Fac Sci, Lab Growth Regulators, Ctr Reg Hana Biotechnol & Agr Res, Olomouc 78371, Czech Republic

[9] Ruhr Univ Bochum, Lehrstuhl Pflanzenphysiol, D-44801 Bochum, Germany

[10] Masaryk Univ, Dept Funct Genom & Prote, Fac Sci, CZ-62500 Brno, Czech Republic

[11] Masaryk Univ, CEITEC, CZ-62500 Brno, Czech Republic

来源：

NATURE COMMUNICATIONS | 2012年 / 3卷

基金：

瑞士国家科学基金会;

关键词：

ENDOPLASMIC-RETICULUM; POLLEN GERMINATION; UDP-GLUCOSE; EXPRESSION; EFFLUX; BIOSYNTHESIS; GRADIENTS; MEMBRANE; PROTEINS; MUTATION;

D O I：

10.1038/ncomms1941

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Auxin is a key coordinative signal required for many aspects of plant development and its levels are controlled by auxin metabolism and intercellular auxin transport. Here we find that a member of PIN auxin transporter family, PIN8 is expressed in male gametophyte of Arabidopsis thaliana and has a crucial role in pollen development and functionality. Ectopic expression in sporophytic tissues establishes a role of PIN8 in regulating auxin homoeostasis and metabolism. PIN8 co-localizes with PIN5 to the endoplasmic reticulum (ER) where it acts as an auxin transporter. Genetic analyses reveal an antagonistic action of PIN5 and PIN8 in the regulation of intracellular auxin homoeostasis and gametophyte as well as sporophyte development. Our results reveal a role of the auxin transport in male gametophyte development in which the distinct actions of ER-localized PIN transporters regulate cellular auxin homoeostasis and maintain the auxin levels optimal for pollen development and pollen tube growth.

引用

页数：9

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