Uptake of ant-derived nitrogen in the myrmecophytic orchid Caularthron bilamellatum

Mutualistic antplant associations are common in a variety of plant families. Some myrmecophytic plants, such as the epiphytic orchid Caularthron bilamellatum, actively form hollow structures that provide nesting space for ants (myrmecodomatia), despite a substantial loss of water-storage tissue. This study aimed at assessing the ability of the orchid to take up nitrogen from ant-inhabited domatia as possible trade-off for the sacrifice of potential water storage capacity. Nitrogen uptake capabilities and uptake kinetics of N-15-labelled compounds (NH4, urea and l -glutamine) were studied in field-grown Caularthron bilamellatum plants in a tropical moist forest in Panama. Plants were either labelled directly, by injecting substrates into the hollow pseudobulbs or indirectly, by labelling of the associated ants in situ. Caularthron bilamellatum plants were able to take up all tested inorganic and organic nitrogen forms through the inner surface of the pseudobulbs. Uptake of NH4 and glutamine followed MichaelisMenten kinetics, but urea uptake was not saturable up to 2 mm. N-15-labelled compounds were rapidly translocated and incorporated into vegetative and reproductive structures. By labelling ants with N-15 in situ, we were able to prove that ants transfer N to the plants under field conditions. Based on N-15 labelling experiments we were able to demonstrate, for the first time, that a myrmecophytic orchid is capable of actively acquiring different forms of nitrogen from its domatia and that nutrient flux from ants to plants does indeed occur under natural conditions. This suggests that beyond anti-herbivore protection host plants benefit from ants by taking up nitrogen derived from ant debris.