Molecular cloning and tumour suppressor function analysis of canine REIC/Dkk-3 in mammary gland tumours

被引：6
作者：
Ochiai, Kazuhiko [1 ]
Watanabe, Masami [2 ]
Azakami, Daigo [1 ]
Michishita, Masaki [3 ]
Yoshikawa, Yasunaga [4 ]
Udagawa, Chihiro [1 ]
Metheenukul, Pornphimon [5 ]
Chahomchuen, Thippayarat [5 ]
Aoki, Hiroshi [1 ]
Kumon, Hiromi [2 ]
Morimatsu, Masami [6 ]
Omi, Toshinori [1 ]
机构：
[1] Nippon Vet & Life Sci Univ, Sch Vet Sci, Dept Vet Nursing & Technol, Tokyo 1808602, Japan
[2] Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Urol, Okayama 7008558, Japan
[3] Nippon Vet & Life Sci Univ, Sch Vet Sci, Dept Vet Pathol, Tokyo 1808602, Japan
[4] Kitasato Univ, Sch Vet Med, Lab Vet Biochem, Aomori 0348628, Japan
[5] Kasetsart Univ, Fac Vet Technol, Dept Vet Technol, Bangkok 10900, Thailand
[6] Hokkaido Univ, Inst Med Genet, Div Dis Model Innovat, Sapporo, Hokkaido 0600815, Japan
来源：
VETERINARY JOURNAL | 2013年 / 197卷 / 03期
基金：
日本学术振兴会;
关键词：
Canine; Mammary gland tumours; REIC/Dkk-3; Tumour suppressor gene; DOWN-REGULATION; GENE; OVEREXPRESSION; APOPTOSIS; CELLS; POPULATION; ACTIVATION; EXPRESSION;
D O I：
10.1016/j.tvjl.2013.04.024
中图分类号：
S85 [动物医学（兽医学）];
学科分类号：
0906 ;
摘要：
REIC/Dkk-3, a member of the human Dickkopf (Dkk) family, plays a role as a suppressor of growth in several human cancers. In this study, the tumour suppression function of canine REIC/Dkk-3 was investigated. The full-length open reading frame of the canine REIC/Dkk-3 homologue was cloned and the tissue distribution of REIC/Dkk-3 mRNA was determined, along with the subcellular localisation of the REIC/Dkk-3 protein in canine cancer cell lines. Expression of REIC/Dkk-3 was lower in mammary gland tumours and in canine mammary carcinoma cell lines than in normal mammary gland tissue. Overexpression of REIC/Dkk-3 induced apoptosis in canine mammary carcinoma cell lines. These results show that expression of REIC/Dkk-3 is downregulated in canine mammary tumours and that one of the functions of this gene is induction of apoptosis. (C) 2013 Elsevier Ltd. All rights reserved.
引用
收藏
页码：769 / 775
页数：7
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