Structural genomics: beyond the Human Genome Project

被引:289
作者
Burley, SK
Almo, SC
Bonanno, JB
Capel, M
Chance, MR
Gaasterland, T
Lin, DW
Sali, A
Studier, FW
Swaminathan, S
机构
[1] Howard Hughes Med Inst, New York, NY 10021 USA
[2] Rockefeller Univ, New York, NY 10021 USA
[3] Albert Einstein Coll Med, Bronx, NY 10461 USA
[4] Brookhaven Natl Lab, Dept Biol, Upton, NY 11973 USA
基金
美国国家卫生研究院;
关键词
D O I
10.1038/13783
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
With access to whole genome sequences for various organisms and imminent completion of the Human Genome Project, the entire process of discovery in molecular and cellular biology is poised to change. Massively parallel measurement strategies promise to revolutionize how we study and ultimately understand the complex biochemical circuitry responsible for controlling normal development, physiologic homeostasis and disease processes. This information explosion is also providing the foundation for an important new initiative in structural biology. We are about to embark on a program of high-throughput X-ray crystallography aimed at developing a comprehensive mechanistic understanding of normal and abnormal human and microbial physiology at the molecular level. We present the rationale for creation of a structural genomics initiative, recount the efforts of ongoing structural genomics pilot studies, and detail the lofty goals, technical challenges and pitfalls facing structural biologists.
引用
收藏
页码:151 / 157
页数:7
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