Spectral Analysis of Methylene Blue-Mediated Photodynamic Inactivation of Bacteria Using a 635-nm Diode Laser System

被引：5

作者：

Calin, Mihaela Antonina ^{[1
]}

Calin, Marian Romeo ^{[2
]}

Savastru, Roxana ^{[1
]}

机构：

[1] Natl Inst Res & Dev Optoelect INOE 2000, Magurele 077125, Ilfov, Romania

[2] Horia Hulubei Natl Inst Phys & Nucl Engn IFIN HH, Magurele, Romania

来源：

SPECTROSCOPY LETTERS | 2013年 / 46卷 / 05期

关键词：

bacteria; laser; photodynamic inactivation; reflectance; spectrum; STAPHYLOCOCCUS-AUREUS; IN-VITRO; PSEUDOMONAS-AERUGINOSA; THERAPY; PHOTOSENSITIZERS; INFECTIONS; EXPOSURE; VIVO; SKIN;

D O I：

10.1080/00387010.2012.730598

中图分类号：

O433 [光谱学];

学科分类号：

0703 ; 070302 ;

摘要：

In this paper we assess the efficacy of a laser system, emitting at 635nm, in methylene bluemediated photodynamic inactivation of Pseudomonas aeruginosa that contaminates wounds, using diffuse reflectance spectrometry. The spectral plots of the contaminated wounds were recorded before and after laser irradiation, and the refractive index and extinction coefficient were determined by Kramers-Kronig analysis of reflectance spectra. The changes in optical parameter values after the treatment were corroborated with bacterial counts determined by bacteriological examination. The results revealed that the combined treatment 635-nm lasermethylene blue is an efficient way to destroy the bacteria that contaminate the wounds.

引用

页码：327 / 333

页数：7

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