Second messenger up-regulation of androgen receptor gene transcription is absent in androgen insensitive human prostatic carcinoma cell lines, PC-3 and DU-145

被引：21
|
作者：
Stubbs, AP
Lalani, EN
Stamp, GWH
Hurst, H
Abel, P
Waxman, J
机构：
[1] HAMMERSMITH HOSP, ROYAL POSTGRAD MED SCH, DEPT HISTOPATHOL, LONDON W12 0NN, ENGLAND
[2] HAMMERSMITH HOSP, ROYAL POSTGRAD MED SCH, DEPT ONCOL, LONDON W12 0NN, ENGLAND
[3] HAMMERSMITH HOSP, ROYAL POSTGRAD MED SCH, DEPT ICRF ONCOL UNIT, LONDON W12 0NN, ENGLAND
[4] HAMMERSMITH HOSP, ROYAL POSTGRAD MED SCH, DEPT SURG, LONDON W12 0NN, ENGLAND
来源：
FEBS LETTERS | 1996年 / 383卷 / 03期
关键词：
androgen receptor; electromobility shift assay; prostate cancer;
D O I：
10.1016/0014-5793(96)00241-4
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A theoretical pathway of transcriptional regulation of the androgen receptor (AR) gene is via a cAMP response element (CRE) present in its promoter region (-508 to -501), After 20 h of stimulation with 8-bromo-cAMP, AR mRNA was upregulated in LNCaP but not in either PC-3 or DU-145 cell lines, We have demonstrated that the level of CRE binding protein (CREB) was the same in all cell lines and that the putative AR-CRE forms specific and competable protein interactions with CREB, The ability to regulate AR gene transcription via the second messenger pathway is lost in the PC-3 and DU-145 cell lines, This may be an important primary mechanism of androgen insensitivity in prostate cancer.
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页码：237 / 240
页数：4
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