Global and Stage Specific Patterns of Kruppel-Associated-Box Zinc Finger Protein Gene Expression in Murine Early Embryonic Cells

被引:43
作者
Corsinotti, Andrea [1 ,2 ]
Kapopoulou, Adamandia [1 ,2 ,3 ]
Gubelmann, Carine [1 ,2 ]
Imbeault, Michael [1 ,2 ]
de Sio, Francesca R. Santoni [1 ,2 ]
Rowe, Helen M. [1 ,2 ]
Mouscaz, Yoann [1 ,2 ,3 ]
Deplancke, Bart [1 ,2 ]
Trono, Didier [1 ,2 ]
机构
[1] Ecole Polytech Fed Lausanne, Sch Life Sci, Lausanne, Switzerland
[2] Ecole Polytech Fed Lausanne, Frontiers Genet Program, Lausanne, Switzerland
[3] Swiss Inst Bioinformat, Lausanne, Switzerland
基金
欧洲研究理事会; 瑞士国家科学基金会;
关键词
TRANSCRIPTIONAL COREPRESSOR TIF1-BETA; GROUND-STATE PLURIPOTENCY; STEM-CELLS; REPRESSION DOMAIN; LEUKEMIA-VIRUS; X-CHROMOSOME; SELF-RENEWAL; HUMAN GENOME; SSX GENES; DIFFERENTIATION;
D O I
10.1371/journal.pone.0056721
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Highly coordinated transcription networks orchestrate the self-renewal of pluripotent stem cell and the earliest steps of mammalian development. KRAB-containing zinc finger proteins represent the largest group of transcription factors encoded by the genomes of higher vertebrates including mice and humans. Together with their putatively universal cofactor KAP1, they have been implicated in events as diverse as the silencing of endogenous retroelements, the maintenance of imprinting and the pluripotent self-renewal of embryonic stem cells, although the genomic targets and specific functions of individual members of this gene family remain largely undefined. Here, we first generated a list of Ensembl-annotated KRAB-containing genes encoding the mouse and human genomes. We then defined the transcription levels of these genes in murine early embryonic cells. We found that the majority of KRAB-ZFP genes are expressed in mouse pluripotent stem cells and other early progenitors. However, we also identified distinctively cell- or stage-specific patterns of expression, some of which are pluripotency-restricted. Finally, we determined that individual KRAB-ZFP genes exhibit highly distinctive modes of expression, even when grouped in genomic clusters, and that these cannot be correlated with the presence of prototypic repressive or activating chromatin marks. These results pave the way to delineating the role of specific KRAB-ZFPs in early embryogenesis.
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页数:16
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