Acinetobacter baumannii Outer Membrane Vesicles Elicit a Potent Innate Immune Response via Membrane Proteins

被引:97
作者
Jun, So Hyun [1 ]
Lee, Jung Hwa [1 ]
Kim, Bo Ra [1 ]
Kim, Seung Il [2 ]
Park, Tae In [3 ]
Lee, Je Chul [1 ]
Lee, Yoo Chul [1 ]
机构
[1] Kyungpook Natl Univ, Sch Med, Dept Microbiol, Taegu, South Korea
[2] Korea Basic Sci Inst, Div Life Sci, Taejon, South Korea
[3] Kyungpook Natl Univ, Sch Med, Dept Pathol, Taegu, South Korea
来源
PLOS ONE | 2013年 / 8卷 / 08期
关键词
PSEUDOMONAS-AERUGINOSA; CELL; BIOGENESIS; PATHOGEN; BIOFILM; ROLES;
D O I
10.1371/journal.pone.0071751
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Acinetobacter baumannii is increasingly becoming a major nosocomial pathogen. This opportunistic pathogen secretes outer membrane vesicles (OMVs) that interact with host cells. The aim of this study was to investigate the ability of A. baumannii OMVs to elicit a pro-inflammatory response in vitro and the immunopathology in response to A. baumannii OMVs in vivo. OMVs derived from A. baumannii ATCC 19606(T) induced expression of pro-inflammatory cytokine genes, interleukin (IL)-beta b and IL-6, and chemokine genes, IL-8, macrophage inflammatory protein-1 alpha, and monocyte chemoattractant protein-1, in epithelial cells in a dose-dependent manner. Disintegration of OMV membrane with ethylenediaminetetraacetic acid resulted in low expression of pro-inflammatory cytokine genes, as compared with the response to intact OMVs. In addition, proteinase K-treated A. baumannii OMVs did not induce significant increase in expression of pro-inflammatory cytokine genes above the basal level, suggesting that the surface-exposed membrane proteins in intact OMVs are responsible for pro-inflammatory response. Early inflammatory processes, such as vacuolization and detachment of epithelial cells and neutrophilic infiltration, were clearly observed in lungs of mice injected with A. baumannii OMVs. Our data demonstrate that OMVs produced by A. baumannii elicit a potent innate immune response, which may contribute to immunopathology of the infected host.
引用
收藏
页数:8
相关论文
共 34 条
  • [11] Do Biofilm Formation and Interactions with Human Cells Explain the Clinical Success of Acinetobacter baumannii?
    de Breij, Anna
    Dijkshoorn, Lenie
    Lagendijk, Ellen
    van der Meer, Joke
    Koster, Abraham
    Bloemberg, Guido
    Wolterbeek, Ron
    van den Broek, Peterhans
    Nibbering, Peter
    [J]. PLOS ONE, 2010, 5 (05):
  • [12] An increasing threat in hospitals:: multidrug-resistant Acinetobacter baumannii
    Dijkshoorn, Lenie
    Nemec, Alexandr
    Seifert, Harald
    [J]. NATURE REVIEWS MICROBIOLOGY, 2007, 5 (12) : 939 - 951
  • [13] Naturally Produced Outer Membrane Vesicles from Pseudomonas aeruginosa Elicit a Potent Innate Immune Response via Combined Sensing of Both Lipopolysaccharide and Protein Components
    Ellis, Terri N.
    Leiman, Sara A.
    Kuehn, Meta J.
    [J]. INFECTION AND IMMUNITY, 2010, 78 (09) : 3822 - 3831
  • [14] Virulence and Immunomodulatory Roles of Bacterial Outer Membrane Vesicles
    Ellis, Terri N.
    Kuehn, Meta J.
    [J]. MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS, 2010, 74 (01) : 81 - +
  • [15] The Acinetobacter baumannii 19606 OmpA Protein Plays a Role in Biofilm Formation on Abiotic Surfaces and in the Interaction of This Pathogen with Eukaryotic Cells
    Gaddy, Jennifer A.
    Tomaras, Andrew P.
    Actis, Luis A.
    [J]. INFECTION AND IMMUNITY, 2009, 77 (08) : 3150 - 3160
  • [16] Acinetobacter baumannii Secretes Cytotoxic Outer Membrane Protein A via Outer Membrane Vesicles
    Jin, Jong Sook
    Kwon, Sang-Oh
    Moon, Dong Chan
    Gurung, Mamata
    Lee, Jung Hwa
    Kim, Seung Il
    Lee, Je Chul
    [J]. PLOS ONE, 2011, 6 (02):
  • [17] Essential role of toll-like receptor 4 in Acinetobacter baumannii-induced immune responses in immune cells
    Kim, Chang-Hwan
    Jeong, Yu-Jin
    Lee, Junglim
    Jeon, Soo-Jin
    Park, Se-Ra
    Kang, Min-Jung
    Park, Jae-Hak
    Park, Jong-Hwan
    [J]. MICROBIAL PATHOGENESIS, 2013, 54 : 20 - 25
  • [18] Serum resistance of Acinetobacter baumannii through the binding of factor H to outer membrane proteins
    Kim, Sang Woo
    Choi, Chul Hee
    Moon, Dong Chan
    Jin, Jong Sook
    Lee, Jung Hwa
    Shin, Ji-Hyun
    Kim, Jung Min
    Lee, Yoo Chul
    Seol, Sung Yong
    Cho, Dong Taek
    Lee, Je Chul
    [J]. FEMS MICROBIOLOGY LETTERS, 2009, 301 (02) : 224 - 231
  • [19] Global gene expression patterns and induction of innate immune response in human laryngeal epithelial cells in response to Acinetobacter baumannii outer membrane protein A
    Kim, Soon Ae
    Yoo, Sung Min
    Hyun, Sung Hee
    Choi, Chul Hee
    Yang, So Young
    Kim, Hwa Jung
    Jang, Byeong Churl
    Il Suh, Seong
    Lee, Je Chul
    [J]. FEMS IMMUNOLOGY AND MEDICAL MICROBIOLOGY, 2008, 54 (01): : 45 - 52
  • [20] Differential roles of CD14 and Toll-like receptors 4 and 2 in murine Acinetobacter pneumonia
    Knapp, S
    Wieland, CW
    Florquin, S
    Pantophlet, R
    Dijkshoorn, L
    Tshimbalanga, N
    Akira, S
    van der Poll, T
    [J]. AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE, 2006, 173 (01) : 122 - 129