Rapamycin mitigates erythrocyte membrane transport functions and oxidative stress during aging in rats

被引：16
作者：
Singh, Abhishek Kumar [1 ]
Singh, Sandeep [1 ]
Garg, Geetika [1 ]
Rizvi, Syed Ibrahim [1 ]
机构：
[1] Univ Allahabad, Dept Biochem, Allahabad 211002, Uttar Pradesh, India
来源：
ARCHIVES OF PHYSIOLOGY AND BIOCHEMISTRY | 2018年 / 124卷 / 01期
关键词：
Aging; erythrocyte membrane; ion transporters; oxidative stress biomarkers; rapamycin; NA+-H+ EXCHANGER; RED-BLOOD-CELLS; ANTIOXIDANT CAPACITY; LIPID-PEROXIDATION; MODULATORY ROLE; NITRIC-OXIDE; LIFE-SPAN; ACID; APOPTOSIS; MODEL;
D O I：
10.1080/13813455.2017.1359629
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Erythrocyte membrane is a suitable model to study various metabolic and physiological functions as it undergoes variety of biochemical changes during aging. An age-dependent modulatory effect of rapamycin on erythrocyte membrane functions is completely unknown. Therefore, the present study was undertaken to investigate the effect of rapamycin on age-dependent impaired activities of transporters/exchangers, altered levels of redox biomarkers, viz. protein carbonyl (PC), lipid hydroperoxides (LHs), total thiol (-SH), sialic acid (SA) and intracellular calcium ion [Ca2+]i, and osmotic fragility of erythrocyte membrane. A significant reduction in membrane-bound activities of Na+/K+-ATPase (NKA) and Ca2+-ATPase (PMCA), and levels of -SH and SA was observed along with a simultaneous induction in Na+/H+ exchanger (NHE) activity and levels of [Ca2+]i, PC, LH and osmotic fragility in old-aged rats. Rapamycin was found to be a promising age-delaying drug that significantly reversed the aging-induced impaired activities of membrane-bound ATPases and altered levels of redox biomarkers.
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收藏
页码：45 / 53
页数：9
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