Canine adipose tissue-derived mesenchymal stem cells ameliorate severe acute pancreatitis by regulating T cells in rats

被引：26

作者：

Kim, Hyun-Wook ^{[1
,2
]}

Song, Woo-Jin ^{[1
]}

Li, Qiang ^{[1
]}

Han, Sei-Myoung ^{[1
]}

Jeon, Kee-Ok ^{[1
]}

Park, Sang-Chul ^{[1
]}

Ryu, Min-Ok ^{[1
]}

Chae, Hyung-Kyu ^{[1
]}

Kyeong, Kweon ^{[1
]}

Youn, Hwa-Young ^{[1
]}

机构：

[1] Seoul Natl Univ, Dept Vet Internal Med, Coll Vet Med, Seoul 08826, South Korea

[2] Haemaru Referral Anim Hosp, Seongnam 13590, South Korea

来源：

JOURNAL OF VETERINARY SCIENCE | 2016年 / 17卷 / 04期

关键词：

acute pancreatitis; anti-inflammatory agents; dogs; mesenchymal stromal cell; regulatory T-lymphocytes; DOGS; INDUCTION; CAPACITY; DAMAGE;

D O I：

10.4142/jvs.2016.17.4.539

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

Severe acute pancreatitis (SAP) is associated with systemic complications and high mortality rate in dogs. Mesenchymal stem cells (MSCs) have been investigated for their therapeutic potential in several inflammation models. In the present study, the effects of canine adipose tissue-derived (cAT)-MSCs in a rat model of SAP induced by retrograde injection of 3% sodium taurocholate solution into the pancreatic duct were investigated. cAT-MSCs labeled with dioctadecyl-3,3,3'-tetramethylindo-carbocyanine perchlorate (1 x 10(7) cells/kg) were systemically administered to rats and pancreatic tissue was collected three days later for histopathological, quantitative real-time polymerase chain reaction, and immunocytochemical analyses. Greater numbers of infused cAT-MSCs were detected in the pancreas of SAP relative to sham-operated rats. cAT-MSC infusion reduced pancreatic edema, inflammatory cell infiltration, and acinar cell necrosis, and decreased pancreatic expression of the pro-inflammatory cytokines tumor necrosis factor-alpha, interleukin (IL)-1 beta, -6, -12, -17, and -23 and interferon-gamma while stimulating expression of the anti-inflammatory cytokines IL-4 and IL-10 in SAP rats. Moreover, cAT-MSCs decreased the number of clusters of differentiation 3-positive T cells and increased that of forkhead box P3-positive T cells in the injured pancreas. These results indicate that cAT-MSCs can be effective as a cell-based therapeutic strategy for treatment of SAP in dogs.

引用

页码：539 / 548

页数：10

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