Subunit D of the vacuolar H+-ATPase of Arabidopsis thaliana

被引：23
|
作者：
Kluge, C [1 ]
Golldack, D [1 ]
Dietz, KJ [1 ]
机构：
[1] Univ Bielefeld, Fac Biol W5, Dept Physiol & Biochem Plants, D-33501 Bielefeld, Germany
来源：
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES | 1999年 / 1419卷 / 01期
关键词：
Arabidopsis thaliana; ATPase; cDNA; in situ hybridization; leaf; subunit D; root; vacuole;
D O I：
10.1016/S0005-2736(99)00055-3
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A 1034 bp cDNA encoding the full length sequence of subunit D of the vacuolar H+-ATPase was cloned from Arabidopsis thaliana. The open reading frame of the cDNA clone vatpD contains 780 bp and codes for a protein of 29.1 kDa with a pi of 9.52. Structural predictions show similarities to subunit gamma of the F-ATP synthases. Identity between subunit D of the vacuolar H+-ATPase of.4. thaliana and subunits D from other eukaryotic organisms is in the range of 57% (Bos taurus) to 48% (Candida albicans). Hybridization of genomic DNA with vatpD indicates the existence of one gene copy of subunit D in A. thaliana. Northern blot hybridization and in situ hybridization showed expression of vatpD in all cell types. The expression of subunit D was not modified by salt stress or abscisic acid treatment in A. thaliana. (C) 1999 Elsevier Science B.V. All rights reserved.
引用
收藏
页码：105 / 110
页数：6
相关论文
共 50 条
  • [1] Subunit C of the vacuolar H+-ATPase of Hordeum vulgare
    Tavakoli, N
    Eckerskorn, C
    Golldack, D
    Dietz, KJ
    FEBS LETTERS, 1999, 456 (01): : 68 - 72
  • [2] Overexpression of a vacuolar H+-pyrophosphatase and a B subunit of H+-ATPase cloned from the halophyte Halostachys caspica improves salt tolerance in Arabidopsis thaliana
    You-zhen Hu
    You-ling Zeng
    Bo Guan
    Fu-chun Zhang
    Plant Cell, Tissue and Organ Culture (PCTOC), 2012, 108 : 63 - 71
  • [3] Overexpression of a vacuolar H+-pyrophosphatase and a B subunit of H+-ATPase cloned from the halophyte Halostachys caspica improves salt tolerance in Arabidopsis thaliana
    Hu, You-zhen
    Zeng, You-ling
    Guan, Bo
    Zhang, Fu-chun
    PLANT CELL TISSUE AND ORGAN CULTURE, 2012, 108 (01) : 63 - 71
  • [4] Transcript level regulation of the vacuolar H+-ATPase subunit isoforms VHA-a, VHA-E and VHA-G in Arabidopsis thaliana
    Hanitzsch, Miriam
    Schnitzer, Daniel
    Seidel, Thorsten
    Golldack, Dortje
    Dietz, Karl-Josef
    MOLECULAR MEMBRANE BIOLOGY, 2007, 24 (5-6) : 507 - 518
  • [5] Vacuolar H+-ATPase subunit VAB3 regulates cell growth and ion homeostasis in Arabidopsis
    Li, Wenbo
    Luo, Laifu
    Gu, Lili
    Li, Haimin
    Zhang, Qian
    Ye, Yajin
    Li, Laigeng
    PLANT JOURNAL, 2022, 112 (03) : 664 - 676
  • [6] Arabidopsis vacuolar H+-ATPase subunit E isoform 1 is required for Golgi organization and vacuole function in embryogenesis
    Strompen, G
    Dettmer, J
    Stierhof, YD
    Schumacher, K
    Jürgens, G
    Mayer, U
    PLANT JOURNAL, 2005, 41 (01) : 125 - 132
  • [7] BIOGENESIS OF THE YEAST VACUOLAR H+-ATPASE
    KANE, PM
    JOURNAL OF EXPERIMENTAL BIOLOGY, 1992, 172 : 93 - 103
  • [8] FUNCTIONAL-ANALYSIS OF CONSERVED CYSTEINE RESIDUES IN THE CATALYTIC SUBUNIT OF THE YEAST VACUOLAR H+-ATPASE
    TAIZ, L
    NELSON, H
    MAGGERT, K
    MORGAN, L
    YATABE, B
    TAIZ, SL
    RUBINSTEIN, B
    NELSON, N
    BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES, 1994, 1194 (02): : 329 - 334
  • [9] SEVERAL DISTINCT GENES ENCODE NEARLY IDENTICAL 16 KDA PROTEOLIPIDS OF THE VACUOLAR H+-ATPASE FROM ARABIDOPSIS-THALIANA
    PERERA, IY
    LI, XH
    SZE, H
    PLANT MOLECULAR BIOLOGY, 1995, 29 (02) : 227 - 244
  • [10] Cloning and molecular characterisation of the trout (Oncorhynchus mykiss) vacuolar H+-ATPase B subunit
    Perry, SF
    Beyers, ML
    Johnson, DA
    JOURNAL OF EXPERIMENTAL BIOLOGY, 2000, 203 (03) : 459 - 470
12345