Subunit D of the vacuolar H+-ATPase of Arabidopsis thaliana

被引：23

作者：

Kluge, C ^{[1
]}

Golldack, D ^{[1
]}

Dietz, KJ ^{[1
]}

机构：

[1] Univ Bielefeld, Fac Biol W5, Dept Physiol & Biochem Plants, D-33501 Bielefeld, Germany

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES | 1999年 / 1419卷 / 01期

关键词：

Arabidopsis thaliana; ATPase; cDNA; in situ hybridization; leaf; subunit D; root; vacuole;

D O I：

10.1016/S0005-2736(99)00055-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A 1034 bp cDNA encoding the full length sequence of subunit D of the vacuolar H+-ATPase was cloned from Arabidopsis thaliana. The open reading frame of the cDNA clone vatpD contains 780 bp and codes for a protein of 29.1 kDa with a pi of 9.52. Structural predictions show similarities to subunit gamma of the F-ATP synthases. Identity between subunit D of the vacuolar H+-ATPase of.4. thaliana and subunits D from other eukaryotic organisms is in the range of 57% (Bos taurus) to 48% (Candida albicans). Hybridization of genomic DNA with vatpD indicates the existence of one gene copy of subunit D in A. thaliana. Northern blot hybridization and in situ hybridization showed expression of vatpD in all cell types. The expression of subunit D was not modified by salt stress or abscisic acid treatment in A. thaliana. (C) 1999 Elsevier Science B.V. All rights reserved.

引用

页码：105 / 110

页数：6

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