Recombination-mediated PCR-directed plasmid construction in vivo in yeast

被引：431

作者：

Oldenburg, KR

Vo, KT

Michaelis, S

Paddon, C

机构：

[1] AFFYMAX RES INST, PALO ALTO, CA 94304 USA

[2] JOHNS HOPKINS UNIV, SCH MED, DEPT CELL BIOL & ANAT, BALTIMORE, MD 21205 USA

来源：

NUCLEIC ACIDS RESEARCH | 1997年 / 25卷 / 02期

关键词：

D O I：

10.1093/nar/25.2.451

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have extended the technique of PCR-directed recombination in Saccharomyces cerevisiae to develop a simple method for plasmid or gene construction in the absence of suitable restriction sites. The DNA to be cloned is PCR-amplified with 30-40 bp of homology to a linearized yeast plasmid. Co-transformation into yeast results in homologous recombination ata position directed by the PCR oligonucleotides.

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页码：451 / 452

页数：2

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