Analysis of Immune Response in Yellowtail upon Vaccination with a Bivalent Vaccine of Vibrio anguillarum and Lactococcus garvieae

被引：6

作者：

Nakajima, Nao ^{[1
,3
]}

Kijima, Mayumi ^{[1
]}

Kawanishi, Michiko ^{[1
]}

Katou, Goushi ^{[2
]}

Futami, Kunihiko ^{[3
]}

Katagiri, Takayuki ^{[3
]}

Endo, Makoto ^{[3
]}

Maita, Masashi ^{[3
]}

机构：

[1] Minist Agr Forestry & Fisheries, Natl Vet Assay Lab, Tokyo 1858511, Japan

[2] Tokyo Univ Marine Sci & Technol, Lab Genome Sci, Tokyo 1088477, Japan

[3] Tokyo Univ Marine Sci & Technol, Lab Fish Hlth Management, Tokyo 1088477, Japan

来源：

FISH PATHOLOGY | 2012年 / 47卷 / 01期

关键词：

Seriola quinqueradiata; Yellowtail; Vaccine; Immune response; Gene expression; Vibrio anguillarum; Lactococcus garvieae; SALMO-GAIRDNERI RICHARDSON; SERIOLA-QUINQUERADIATA; RAINBOW-TROUT; GADUS-MORHUA; JAPANESE FLOUNDER; EXPRESSION; MUCUS; FISH; COD; IMMUNOGLOBULIN;

D O I：

10.3147/jsfp.47.12

中图分类号：

S9 [水产、渔业];

学科分类号：

0908 ;

摘要：

We inoculated yellowtail Seriola quinqueradiata with a commercial bivalent vaccine containing inactivated Vibrio anguillarum and Lactococcus garvieae to better understand the immunological responses to the vaccination. Serum samples were collected from vaccinated and control (PBS-injected) fish at 1, 3, 5, 7, 10, and 14 days post-vaccination (dpv). Compared to the controls, the serum from the vaccinated fish inhibited the proliferation of V anguillarum from 7 dpv. However, vaccination had no effect on L. garvieae proliferation. The antibody titer against V anguillarum obviously increased from 7 dpv, but no such increase was detected for L. garvieae. We confirmed that the protection conferred by the V anguillarum vaccine was a result of the antibodies against the pathogen. However, humoral factors related to protection were not observed for L. garvieae. The expression of immune-related genes in the head kidney was profiled by microarray analysis. Twenty-two immune-related genes were up-regulated during the experimental period. Up-regulation of cellular immune-related genes, such as tumor necrosis factor, CC chemokine, and macrophage colony-stimulating factor receptor, was mostly observed from 1 to 3 dpv, whereas that of humoral immune-related genes was observed from 3 to 14 dpv. Notably, immunoglobulin-related genes showed continuous up-regulation during the 2-week experimental period.

引用

页码：12 / 19

页数：8

共 41 条

[1] LPS increases the expression levels of IL-18, ICE and IL-18 R in mouse testes [J].

Abu Elhija, Mahmoud ;

Lunenfeld, Eitan ;

Huleihel, Mahmoud .

AMERICAN JOURNAL OF REPRODUCTIVE IMMUNOLOGY, 2008, 60 (04) :361-371

[2]

Alexander John B., 1992, Annual Review of Fish Diseases, V2, P249, DOI 10.1016/0959-8030(92)90066-7

[3] Epidermal proteases of the Japanese eel [J].

Aranishi, F ;

Nakane, M .

FISH PHYSIOLOGY AND BIOCHEMISTRY, 1997, 16 (06) :471-478

[4] HSP70 stimulates cytokine production through a CD14-dependant pathway, demonstrating its dual role as a chaperone and cytokine [J].

Asea, A ;

Kraeft, SK ;

Kurt-Jones, EA ;

Stevenson, MA ;

Chen, LB ;

Finberg, RW ;

Koo, GC ;

Calderwood, SK .

NATURE MEDICINE, 2000, 6 (04) :435-442

[5]

Bengten E., 1994, COMP IMMUNOL, V15, P143

[6] Intraperitoneal vaccination of Atlantic cod, Gadus morhua with heat-killed Listonella anguillarum enhances serum antibacterial activity and expression of immune response genes [J].

Caipang, Christopher Marlowe A. ;

Hynes, Natasha ;

Puangkaew, Jumroensri ;

Brinchmann, Monica F. ;

Kiron, Viswanath .

FISH & SHELLFISH IMMUNOLOGY, 2008, 24 (03) :314-322

[7] Profiling gene expression in the spleen of Atlantic cod, Gadus morhua upon vaccination with Vibrio anguillarum antigen [J].

Caipang, Christopher Marlowe A. ;

Brinchmann, Monica F. ;

Kiron, Viswanath .

COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY, 2009, 153 (03) :261-267

[8] Immune-related gene expression profiling of yellowtail (Seriola quinqueradiata) kidney cells stimulated with ConA and LPS using microarray analysis [J].

Darawiroj, Damri ;

Kondo, Hidehiro ;

Hirono, Ikuo ;

Aoki, Takashi .

FISH & SHELLFISH IMMUNOLOGY, 2008, 24 (02) :260-266

[9] Innate host defense mechanisms of fish against viruses and bacteria [J].

Ellis, AE .

DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY, 2001, 25 (8-9) :827-839

[10] VACCINATION EXPERIMENTS AND STUDIES OF THE HUMORAL IMMUNE-RESPONSES IN COD, GADUS-MORHUA L, TO 4 STRAINS OF MONOCLONAL-DEFINED VIBRIO-ANGUILLARUM [J].

ESPELID, S ;

RODSETH, OM ;

JORGENSEN, TO .

JOURNAL OF FISH DISEASES, 1991, 14 (02) :185-197

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