The Large Subunit rDNA Sequence of Plasmodiophora brassicae Does not Contain Intra-species Polymorphism

被引：21

作者：

Schwelm, Arne ^{[1
,2
]}

Berney, Cedric ^{[3
,4
,5
]}

Dixelius, Christina ^{[1
]}

Bass, David ^{[3
,6
]}

Neuhauser, Sigrid ^{[2
,3
]}

机构：

[1] Swedish Univ Agr Sci, Dept Plant Biol, Uppsala BioCtr, Linnean Ctr Plant Biol, POB 7080, SE-75007 Uppsala, Sweden

[2] Univ Innsbruck, Inst Microbiol, Technikerstr 25, A-6020 Innsbruck, Austria

[3] Nat Hist Museum London, Dept Life Sci, Div Genom & Microbial Divers, Cromwell Rd, London SW7 5BD, England

[4] CNRS, UMR 7144, Grp Evolut Protistes & Ecosyst Pelag, Pl Georges Teissier, F-29680 Roscoff, France

[5] Univ Paris 06, Stn Biol Roscoff, Pl Georges Teissier, F-29680 Roscoff, France

[6] Ctr Environm Fisheries & Aquaculture Sci Cefas, Barrack Rd, The Nothe DT4 8UB, Weymouth, England

来源：

PROTIST | 2016年 / 167卷 / 06期

基金：

奥地利科学基金会;

关键词：

Clubroot; FISH (fluorescence in-situ hybridisation); LSU rDNA; pathotyping; phytomyxea; plasmodiophorids; VIRULENCE; PHYLOGENY; DIVERSITY; INSIGHTS; CLUBROOT; REVEALS; CABBAGE;

D O I：

10.1016/j.protis.2016.08.008

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Clubroot disease caused by Plasmodiophora brassicae is one of the most important diseases of cultivated brassicas. P. brassicae occurs in pathotypes which differ in the aggressiveness towards their Brassica host plants. To date no DNA based method to distinguish these pathotypes has been described. In 2011 polymorphism within the 28S rDNA of P. brassicae was reported which potentially could allow to distinguish pathotypes without the need of time-consuming bioassays. However, isolates of P. brassicae from around the world analysed in this study do not show polymorphism in their LSU rDNA sequences. The previously described polymorphism most likely derived from soil inhabiting Cercozoa more specifically Neoheteromita-like glissomonads. Here we correct the LSU rDNA sequence of P. brassicae. By using FISH we demonstrate that our newly generated sequence belongs to the causal agent of clubroot disease. (C) 2016 The Authors. Published by Elsevier GmbH.

引用

页码：544 / 554

页数：11

共 26 条

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