Differential Effects of Flavonoids on Barrier Integrity in Human Intestinal Caco-2 Cells

被引：104

作者：

Noda, Sakino ^{[1
]}

Tanabe, Soichi ^{[1
]}

Suzuki, Takuya ^{[1
]}

机构：

[1] Hiroshima Univ, Grad Sch Biosphere Sci, Dept Biofunct Sci & Technol, Higashihiroshima 7398528, Japan

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2012年 / 60卷 / 18期

基金：

日本学术振兴会;

关键词：

flavonoids; tight junction; intestinal barrier; Caco-2; cells; DIETARY POLYPHENOLS; EPITHELIAL-CELLS; TIGHT JUNCTIONS; CROHNS-DISEASE; OCCLUDIN; PERMEABILITY; EXPRESSION; CLAUDIN-1; PROTEINS; HEALTH;

D O I：

10.1021/jf300382h

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

Flavonoids, present in fruits, vegetables, and teas, provide beneficial effects for our health. We investigated the effect of a number of flavonoids on tight junction (TJ) barrier integrity in human intestinal Caco-2 cells. Transepithelial electrical resistance (TER; a TJ integrity marker) across cell monolayers was measured in cells incubated with flavonoids for 24 h. Chrysin decreased the TER, indicating a decrease in TJ integrity. Daidzein, hesperetin, naringenin, and morin increased the TER, indicating increased TJ integrity. Luteolin and genistein increased or normalized the TER after a transient decrease. Immunoblot analysis revealed that these changes in TER were caused by modification of the cytoskeletal association and expression of TJ proteins, zonula occludens (ZO)-1, ZO-2, occludin, junctional adhesion molecule-1, and/or claudins. Our results suggest that various flavonoids participate in the regulation of intestinal TJ barrier integrity and that this regulation may partially contribute to the flavonoid-mediated biological effects on our health.

引用

页码：4628 / 4633

页数：6

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