Genetic conservation of potentially immunogenic proteins among Brazilian isolates of Babesia bovis

被引：22
作者：
Ramos, Carlos A. N. [1 ,2 ]
Araujo, Flabio R. [1 ]
Alves, Leucio C. [2 ]
Fernando de Souza, Ingrid Leda [1 ,3 ]
Guedes, Daniel S., Jr. [4 ]
Soares, Cleber Oliveira [1 ]
机构：
[1] Embrapa Gado Corte, BR-79002970 Campo Grande, MS, Brazil
[2] Univ Fed Rural Pernambuco, Dept Vet Med, Lab Doencas Parasitarias Anim Domest, BR-52171900 Recife, PE, Brazil
[3] Univ Fed Mato Grosso do Sul, BR-79070900 Campo Grande, MS, Brazil
[4] Fiocruz MS, BR-21040360 Rio De Janeiro, RJ, Brazil
来源：
VETERINARY PARASITOLOGY | 2012年 / 187卷 / 3-4期
关键词：
Genetic conservation; Babesia bovis; Vaccine; Brazil; RHOPTRY-ASSOCIATED PROTEIN-1; B-CELL EPITOPES; MEROZOITE; SURFACE; SEQUENCE; ANTIBODIES; CHALLENGE; RESPONSES; ANTIGENS; VACCINES;
D O I：
10.1016/j.vetpar.2012.01.020
中图分类号：
R38 [医学寄生虫学]; Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ; 100103 ;
摘要：
Bovine babesiosis caused by Babesia bovis remains an important constraint for the development of cattle industries worldwide. Effective control can be achieved by vaccination with live attenuated phenotypes of the parasite. However, these vaccines have a number of drawbacks, which justifies the search for better, safer vaccines. In recent years, a number of parasite proteins with immunogenic potential have been discovered. However, there is little information on the genetic conservation of these proteins among different parasite isolates, which hinders their assessment as immunogens. The aim of the present study was to evaluate the conservation of the genes ama-1, acs-1, rap-1, trap, p0 and msa2c among five Brazilian isolates of B. bovis. Through polymerase chain reaction, genetic sequencing and bioinformatics analysis of the genes, a high degree of conservation (98-100%) was found among Brazilian isolates of B. bovis and the T2Bo isolate. Thus, these genes are worth considering as viable candidates to be included in a recombinant cocktail vaccine for cattle babesiosis caused by B. bovis. (C) 2012 Elsevier B.V. All rights reserved.
引用
收藏
页码：548 / 552
页数：5
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