Characterization of expression and enzyme activity of lipoxygenases during fruit softening and superficial scald development in 'Wujiuxiang' pear

被引：5
作者：
Zhou, Shuo [1 ,2 ]
Li, Dan [1 ,2 ]
Cheng, Yudou [1 ,2 ]
Guan, Junfeng [1 ,2 ]
机构：
[1] Hebei Acad Agr & Forestry Sci, Inst Genet & Physiol, Shijiazhuang 050051, Peoples R China
[2] Plant Genet Engn Ctr Hebei Prov, Shijiazhuang, Peoples R China
来源：
JOURNAL OF APPLIED BOTANY AND FOOD QUALITY | 2016年 / 89卷
关键词：
LOX GENE FAMILY; ETHYLENE; STRESS; IDENTIFICATION; SENESCENCE; TOMATO; ACID; 1-METHYLCYCLOPROPENE; BIOSYNTHESIS; REGENERATION;
D O I：
10.5073/JABFQ.2016.089.040
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
Lipoxygenases (LOXs; EC 1.13.11.12) have been implicated in fruit r ipening and senescence; however, little is known regarding the specific LOX genes involved in fruit softening and scald development in pear. In this study, two 9-lipoxygenase genes, PcLOX1 and PcLOX7, were characterized in silico in the Pyrus communis draft genome. The expression pattern of PcLOX1 and PcLOX7, LOX activity, respiration rate and ethylene production were analyzed during fruit softening and superficial scald development in 'Wujiuxiang' pear with or without 1-MCP treatment. The results demonstrated that PcLOX1 and PcLOX7 belong to the type-1 LOX subfamily, and their expression levels, enzyme activity, respiration rate and ethylene production were increased during fruit softening and scald development. In contrast, 1-MCP treatment could effectively suppress respiration rate and ethylene production and inhibit expression of PcLOX1 and PcLOX7 and LOX enzyme activity, resulting in delayed fruit softening and scald development. These findings indicate that PcLOX1 and PcLOX7 are involved in fruit ripening and scald development in 'Wujiuxiang' pear.
引用
收藏
页码：307 / 314
页数：8
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