Spectrophotometric investigation of the hetero-association of Caffeine and thiazine dye in aqueous solution

被引：34
作者：
Bolotin, P. A. [1 ]
Baranovsky, S. F. [1 ]
Evstigneev, M. P. [1 ]
机构：
[1] Sevastopol Natl Tech Univ, Dept Phys, Sevastopol 99053, Crimea, Ukraine
来源：
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY | 2006年 / 64卷 / 03期
关键词：
methylene Blue; caffeine; spectrophotometry; thermodynamics; hetero-association; self-association;
D O I：
10.1016/j.saa.2005.08.005
中图分类号：
O433 [光谱学];
学科分类号：
0703 ; 070302 ;
摘要：
The self-association of thiazine dye, Methylene Blue (MB), and its hetero-association with Caffeine (CAF), were studied in aqueous solution by means of spectrophotometry in the visible range of spectrum. Concentration and temperature dependences of molar absorption of the interacting molecules were used to analyse dynamic equilibrium in solution in terms of two-component model of molecular hetero-association. The magnitudes of equilibrium dimerization and hetero-association constants as well as thermodynamic parameters, enthalpy and entropy, were determined. The calculation of the fraction of different types of associates in the mixed solution, containing Methylene Blue and Caffeine, was done. It was concluded that the hetero-association of Methylene Blue and Caffeine molecules results in lower effective concentration of the dye in solution, which may account for the alteration of its biological activity. (c) 2005 Elsevier B.V. All rights reserved.
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页码：693 / 697
页数：5
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