Hydroxybenzotriazole increases the range of textile dyes decolorized by immobilized laccase

被引：110

作者：

Reyes, P

Pickard, MA

Vazquez-Duhalt, R

机构：

[1] Univ Nacl Autonoma Mexico, Inst Biotecnol, Cuernavaca 62250, Morelos, Mexico

[2] Univ Alberta, Dept Biol Sci, Edmonton, AB T6G 2E9, Canada

来源：

BIOTECHNOLOGY LETTERS | 1999年 / 21卷 / 10期

关键词：

dye; immobilized enzyme; laccase; mediators; textile wastewater;

D O I：

10.1023/A:1005502906890

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Laccase from Coriolopsis gallica UAMH8260 was immobilized on activated agarose and tested for repeated decolorization of industrial dyes. Immobilized enzyme retained 85% of the initial activity after 10 cycles, and 70% after 3 months of intermittant use in the decolorization of Reactive Blue 198 dye. Free laccase decolorized 13 of 38 industrial dyes tested but, in the presence of 1 mM 1-hydroxybenzotriazole as a free radical mediator, the enzyme decolorized 26 of the 38 dyes increasing both the range and rate of decolorization. Immobilized laccase showed a higher thermal stability at 70 degrees C than free enzyme but no increased resistance to organic solvents.

引用

页码：875 / 880

页数：6

共 37 条

[1] DECOLORIZATION OF KRAFT BLEACHERY EFFLUENT CHROMOPHORES BY CORIOLUS-(TRAMETES)-VERSICOLOR [J].