Break-induced replication occurs by conservative DNA synthesis

被引:140
作者
Donnianni, Roberto A. [1 ]
Symington, Lorraine S. [1 ]
机构
[1] Columbia Univ, Dept Microbiol & Immunol, Med Ctr, New York, NY 10032 USA
基金
美国国家卫生研究院;
关键词
translocation; Pol32; cell cycle; HOMOLOGOUS RECOMBINATION; SACCHAROMYCES-CEREVISIAE; HALF-CROSSOVERS; REPAIR; YEAST; TRANSLOCATIONS; GENES; RAD51; LEADS; SRS2;
D O I
10.1073/pnas.1309800110
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Break-induced replication (BIR) refers to recombination-dependent DNA synthesis initiated from one end of a DNA double-strand break and can extend for more than 100 kb. BIR initiates by Rad51-catalyzed strand invasion, but the mechanism for DNA synthesis is not known. Here, we used BrdU incorporation to track DNA synthesis during BIR and found that the newly synthesized strands segregate with the broken chromosome, indicative of a conservative mode of DNA synthesis. Furthermore, we show the frequency of BIR is reduced and product formation is progressively delayed when the donor is placed at an increasing distance from the telomere, consistent with replication by a migrating D-loop from the site of initiation to the telomere.
引用
收藏
页码:13475 / 13480
页数:6
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