Break-induced replication (BIR) refers to recombination-dependent DNA synthesis initiated from one end of a DNA double-strand break and can extend for more than 100 kb. BIR initiates by Rad51-catalyzed strand invasion, but the mechanism for DNA synthesis is not known. Here, we used BrdU incorporation to track DNA synthesis during BIR and found that the newly synthesized strands segregate with the broken chromosome, indicative of a conservative mode of DNA synthesis. Furthermore, we show the frequency of BIR is reduced and product formation is progressively delayed when the donor is placed at an increasing distance from the telomere, consistent with replication by a migrating D-loop from the site of initiation to the telomere.
机构:Univ Calif San Diego, Sch Med, Ludwig Inst Canc Res, Ctr Canc, La Jolla, CA 92093 USA
Chen, C
;
Kolodner, RD
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Univ Calif San Diego, Sch Med, Ludwig Inst Canc Res, Ctr Canc, La Jolla, CA 92093 USAUniv Calif San Diego, Sch Med, Ludwig Inst Canc Res, Ctr Canc, La Jolla, CA 92093 USA
机构:
Columbia Univ, Dept Biol Sci, New York, NY 10027 USA
Columbia Univ Coll Phys & Surg, Dept Microbiol & Immunol, New York, NY 10032 USAColumbia Univ, Dept Biol Sci, New York, NY 10027 USA
Chen, Huan
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机构:
Lisby, Michael
;
Symington, Lorraine S.
论文数: 0引用数: 0
h-index: 0
机构:
Columbia Univ Coll Phys & Surg, Dept Microbiol & Immunol, New York, NY 10032 USAColumbia Univ, Dept Biol Sci, New York, NY 10027 USA
机构:
CORNELL UNIV MED COLL,DEPT MICROBIOL,HEARST MICROBIOL RES CTR,NEW YORK,NY 10021CORNELL UNIV MED COLL,DEPT MICROBIOL,HEARST MICROBIOL RES CTR,NEW YORK,NY 10021
Ferguson, DO
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Holloman, WK
论文数: 0引用数: 0
h-index: 0
机构:
CORNELL UNIV MED COLL,DEPT MICROBIOL,HEARST MICROBIOL RES CTR,NEW YORK,NY 10021CORNELL UNIV MED COLL,DEPT MICROBIOL,HEARST MICROBIOL RES CTR,NEW YORK,NY 10021
机构:Cornell Univ, Weill Grad Sch Med Sci, Program Mol Biol, New York, NY 10021 USA
Heller, RC
;
Marians, KJ
论文数: 0引用数: 0
h-index: 0
机构:
Cornell Univ, Weill Grad Sch Med Sci, Program Mol Biol, New York, NY 10021 USACornell Univ, Weill Grad Sch Med Sci, Program Mol Biol, New York, NY 10021 USA
机构:Univ Calif San Diego, Sch Med, Ludwig Inst Canc Res, Ctr Canc, La Jolla, CA 92093 USA
Chen, C
;
Kolodner, RD
论文数: 0引用数: 0
h-index: 0
机构:
Univ Calif San Diego, Sch Med, Ludwig Inst Canc Res, Ctr Canc, La Jolla, CA 92093 USAUniv Calif San Diego, Sch Med, Ludwig Inst Canc Res, Ctr Canc, La Jolla, CA 92093 USA
机构:
Columbia Univ, Dept Biol Sci, New York, NY 10027 USA
Columbia Univ Coll Phys & Surg, Dept Microbiol & Immunol, New York, NY 10032 USAColumbia Univ, Dept Biol Sci, New York, NY 10027 USA
Chen, Huan
;
论文数: 引用数:
h-index:
机构:
Lisby, Michael
;
Symington, Lorraine S.
论文数: 0引用数: 0
h-index: 0
机构:
Columbia Univ Coll Phys & Surg, Dept Microbiol & Immunol, New York, NY 10032 USAColumbia Univ, Dept Biol Sci, New York, NY 10027 USA
机构:
CORNELL UNIV MED COLL,DEPT MICROBIOL,HEARST MICROBIOL RES CTR,NEW YORK,NY 10021CORNELL UNIV MED COLL,DEPT MICROBIOL,HEARST MICROBIOL RES CTR,NEW YORK,NY 10021
Ferguson, DO
;
Holloman, WK
论文数: 0引用数: 0
h-index: 0
机构:
CORNELL UNIV MED COLL,DEPT MICROBIOL,HEARST MICROBIOL RES CTR,NEW YORK,NY 10021CORNELL UNIV MED COLL,DEPT MICROBIOL,HEARST MICROBIOL RES CTR,NEW YORK,NY 10021
机构:Cornell Univ, Weill Grad Sch Med Sci, Program Mol Biol, New York, NY 10021 USA
Heller, RC
;
Marians, KJ
论文数: 0引用数: 0
h-index: 0
机构:
Cornell Univ, Weill Grad Sch Med Sci, Program Mol Biol, New York, NY 10021 USACornell Univ, Weill Grad Sch Med Sci, Program Mol Biol, New York, NY 10021 USA