ALG-2 knockdown in HeLa cells results in G2/M cell cycle phase accumulation and cell death

被引：33
作者：
Hoj, Berit R. [1 ]
la Cour, Jonas M. [1 ]
Mollerup, Jens [1 ]
Berchtold, Martin W. [1 ]
机构：
[1] Univ Copenhagen, Dept Biol, Copenhagen Bioctr, DK-2200 Copenhagen N, Denmark
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2009年 / 378卷 / 01期
关键词：
Calcium-binding proteins; Apoptosis-linked gene-2 (ALG-2); siRNA; Apoptosis; Cell cycle; Caspase; APOPTOSIS-LINKED GENE-2; RETICULUM EXIT SITES; PROTEIN ALG-2; MITOTIC CATASTROPHE; LOCALIZATION; EXPRESSION; SEC31A;
D O I：
10.1016/j.bbrc.2008.11.021
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
ALG-2 (apoptosis-linked gene-2 encoded protein) has been shown to be upregulated in a variety of human tumors questioning its previously assumed pro-apoptotic function. The aim of the present study was to obtain insights into the role of ALG-2 in human cancer cells. We show that ALG-2 downregulation induces accumulation of HeLa cells in the G2/M cell cycle phase and increases the amount of early apoptotic and dead cells. Caspase inhibition by the pan-caspase inhibitor zVAD-fmk attenuated the increase in the amount of dead cells following ALG-2 downregulation. Thus, our results indicate that ALG-2 has an anti-apoptotic function in HeLa cells by-facilitating the passage through checkpoints in the G2/M cell cycle phase (C) 2008 Elsevier Inc. All rights reserved.
引用
收藏
页码：145 / 148
页数：4
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