Characterization of Streptococcus suis through serotyping, SE-AFLP and virulence profile

被引：2

作者：

Calderaro, Franco F. ^{[1
]}

Moreno, Luisa Z. ^{[1
]}

Doto, Daniela S. ^{[1
]}

Matajira, Carlos E. C. ^{[1
]}

Gomes, Vasco T. M. ^{[1
]}

Ferreira, Thais S. P. ^{[1
]}

Mesquita, Renan E. ^{[1
]}

Moreno, Andrea M. ^{[1
]}

机构：

[1] Univ Sao Paulo, FMVZ, Lab Sanidade Suina & Virol, Ave Prof Dr Orlando Marques de Paiva 87, BR-05508270 Sao Paulo, SP, Brazil

来源：

PESQUISA VETERINARIA BRASILEIRA | 2016年 / 36卷 / 08期

基金：

巴西圣保罗研究基金会;

关键词：

Streptococcus suis; serotyping; SE-AFLP; genotype; virulence genes; FRAGMENT LENGTH POLYMORPHISM; GENETIC DIVERSITY; CAPSULAR TYPES; DISEASED PIGS; STRAINS; SUILYSIN; PARANA; EF;

D O I：

10.1590/S0100-736X2016000800004

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

Streptococcus suis is one of most important pathogens in the swine industry worldwide. Despite its importance, studies of S. suis characterization in South America are still rare. This study evaluates S. suis isolates from distinct Brazilian states, from 1999 to 2004, and its molecular and serological characterization. A total of 174 isolates were studied. S. suis identification was confirmed by PCR and isolates were further serotyped and genotyped by SE-AFLP and amplification of virulence markers. Serotype 1, 2, 3, 4, 7, 18, 22 and 32 were identified among the studied isolates, and only 4% were characterized as non-typeable. The mrp(+)/epf(+)/sly(+) genotype was the most frequent. The SE-AFLP analysis resulted in 29 patterns distributed in three main clusters with over 65% of genetic similarity. Isolates presented a slight tendency to cluster according to serotype and origin; however, no further correlation with virulence genotypes was observed.

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页码：701 / 704

页数：4

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