Automated database-guided expert-supervised orientation for immunophenotypic diagnosis and classification of acute leukemia

被引：50

作者：

Lhermitte, L. ^{[1
,2
]}

Mejstrikova, E. ^{[3
]}

van der Sluijs-Gelling, A. J. ^{[4
,5
]}

Grigore, G. E. ^{[6
]}

Sedek, L. ^{[7
]}

Bras, A. E. ^{[8
]}

Gaipa, G. ^{[9
]}

da Costa, E. Sobral ^{[10
]}

Novakova, M. ^{[3
]}

Sonneveld, E. ^{[4
]}

Buracchi, C. ^{[8
]}

de Sa Bacelar, T. ^{[10
]}

Marvelde, J. G. te ^{[8
]}

Trinquand, A. ^{[1
]}

Asnafi, V. ^{[1
]}

Szczepanski, T. ^{[11
]}

Matarraz, S. ^{[12
,13
,14
]}

Lopez, A. ^{[12
,13
,14
]}

Vidriales, B. ^{[15
]}

Bulsa, J. ^{[11
]}

Hrusak, O. ^{[3
]}

Kalina, T. ^{[3
]}

Lecrevisse, Q. ^{[12
,13
,14
]}

Ayuso, M. Martin ^{[6
]}

Brueggemann, M. ^{[16
]}

Verde, J. ^{[6
]}

Fernandez, P. ^{[17
]}

Burgos, L. ^{[18
]}

Paiva, B. ^{[18
]}

Pedreira, C. E. ^{[19
]}

van Dongen, J. J. M. ^{[5
]}

Orfao, A. ^{[12
,13
,14
]}

van der Velden, V. H. J. ^{[8
]}

机构：

[1] Univ Paris 05, Sorbonne Paris Cite, Inst Necker Enfants Malades, INSERM,UMR1151, Paris, France

[2] Necker Enfants Malades, AP HP, Biol Hematol, Paris, France

[3] Charles Univ Prague, Univ Hosp Motol, Dept Paediat Haematol & Oncol, CLIP,Fac Med 2, Prague, Czech Republic

[4] Dutch Childhood Oncol Grp, The Hague, Netherlands

[5] Leiden Univ, Med Ctr, Dept Immunohematol & Blood Transfus IHB, Leiden, Netherlands

[6] Cytognos SL, Salamanca, Spain

[7] Med Univ Silesia SUM, Dept Microbiol & Immunol, Katowice, Poland

[8] Univ Med Ctr Rotterdam, Erasmus MC, Dept Immunol, Rotterdam, Netherlands

[9] Univ Milano Bicocca, Pediat Clin, Tettamanti Res Ctr, Monza, MB, Italy

[10] Univ Fed Rio de Janeiro, Fac Med, Dept Pediat, Rio De Janeiro, Brazil

[11] Med Univ Silesia SUM, Dept Pediat Hematol & Oncol, Katowice, Poland

[12] Univ Salamanca USAL, Inst Biomed Res Salamanca IBSAL, Canc Res Ctr IBMCC CSIC, Dept Med, Salamanca, Spain

[13] Univ Salamanca USAL, Inst Biomed Res Salamanca IBSAL, Cytometry Serv, Salamanca, Spain

[14] Inst Carlos III, Ctr Invest Biomed Red Canc, Madrid, Spain

[15] Univ Hosp Salamanca, Hematol Serv, Salamanca, Spain

[16] Univ Schleswig Holstein, Dept Hematol, Campus Kiel, Kiel, Germany

[17] Kantonsspital Aarau AG, Inst Lab Med, Aarau, Switzerland

[18] Univ Navarra, CIMA, Clin Univ Navarra, Pamplona, Spain

[19] Univ Fed Rio de Janeiro, COPPE Computat Engn & Syst Postgrad, Rio De Janeiro, Brazil

来源：

LEUKEMIA | 2018年 / 32卷 / 04期

关键词：

ACUTE LYMPHOBLASTIC-LEUKEMIA; FLOW-CYTOMETRY; STANDARDIZATION; PROTOCOLS; LINEAGE;

D O I：

10.1038/leu.2017.313

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Precise classification of acute leukemia (AL) is crucial for adequate treatment. EuroFlow has previously designed an AL orientation tube (ALOT) to guide towards the relevant classification panel (T-cell acute lymphoblastic leukemia (T-ALL), B-cell precursor (BCP)-ALL and/or acute myeloid leukemia (AML)) and final diagnosis. Now we built a reference database with 656 typical AL samples (145 T-ALL, 377 BCP-ALL, 134 AML), processed and analyzed via standardized protocols. Using principal component analysis (PCA)-based plots and automated classification algorithms for direct comparison of single-cells from individual patients against the database, another 783 cases were subsequently evaluated. Depending on the database-guided results, patients were categorized as: (i) typical T, B or Myeloid without or; (ii) with a transitional component to another lineage; (iii) atypical; or (iv) mixed-lineage. Using this automated algorithm, in 781/783 cases (99.7%) the right panel was selected, and data comparable to the final WHO-diagnosis was already provided in 493% of cases (85% T-ALL, 97% BCP-ALL, 95% AML and 87% mixed-phenotype AL patients), even without data on the full-characterization panels. Our results show that database-guided analysis facilitates standardized interpretation of ALOT results and allows accurate selection of the relevant classification panels, hence providing a solid basis for designing future WHO AL classifications.

引用

页码：874 / 881

页数：8

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