DEVELOPMENT AND VALIDATION OF STABILITY INDICATING UPLC METHOD FOR THE SIMULTANEOUS ESTIMATION OFBENZOIC ACID,SALICYLIC ACID,TRIAMCINOLONE ACETONIDE, ICHTHAMOL IN BULK AND PHARMACEUTICAL DOSAGE FORM

A Simple, accurate, rapid and precise Ultra performance liquid chromatographic method has been developed and validated for the determination of Benzoic acid, Salicylic acid, Triamcinolone acetonide and Ichthammol. The chromatographic separation was done on isocratic elution using pentafluorophenyl column (150x4.6mm, 3 mu). Flow rate is 1mL/min and UV detection was carried out at 278nm. The mobile phase consisted by Octane Sulphonic acid with addition of 1ml triehtyl amine and adjusts pH7.0 with ortho phosphoric acid: Acetonitrile in 70:30 % V/V. The retention time for Benzoic acid, Salicylic acid, Triamcinolone acetonide and Ichthammol were found to be 2.54, 5.24, 8.62, 7.77 min respectively. The detector was showed linear response over the concentration range of 60-900 mu g/ml of Benzoic acid, 30450 mu g/ml of Salicylic acid, 20-300 mu g/ml of Ichthammol and 0.1-1.5 mu g/ml of Triamcinolone acetonide for the drugs by showing a good correlation coefficient of 0.999. This proposed method is highly sensitive, precise and accurate which reduces cost of analysis, hence recommended for routine quality analysis in laboratories.