DNA vaccine encoding the major virulence factors of Shiga toxin type 2e (Stx2e)-expressing Escherichia coli induces protection in mice

被引：18

作者：

Ren, Wenkai ^{[1
,3
]}

Yu, Rui ^{[2
]}

Liu, Gang ^{[1
,4
]}

Li, Nengzhang ^{[3
]}

Peng, Yuanyi ^{[3
]}

Wu, Miaomiao ^{[1
]}

Yin, Yulong ^{[1
]}

Li, Yinghui ^{[1
]}

Fatufe, Andrew A. ^{[1
]}

Li, Teijun ^{[1
]}

机构：

[1] Chinese Acad Sci, Sci Observing & Expt Stn Anim Nutr & Feed Sci S C, Minist Agr,Hunan Prov Engn Res Ctr Hlth Livestock, Key Lab Agroecol Proc Subtrop Reg,Inst Subtrop Ag, Changsha 410125, Hunan, Peoples R China

[2] Beijing Inst Biotechnol, Beijing 100071, Peoples R China

[3] Southwest Univ, Coll Anim Sci & Technol, Chongqing Key Lab Forage & Herbivorce, Chongqing 400716, Peoples R China

[4] Chinese Acad Sci, Inst Microbiol, State Key Lab Microbial Resources, Changsha 410125, Hunan, Peoples R China

来源：

VACCINE | 2013年 / 31卷 / 02期

关键词：

Stx2eB; FedF; Escherichia coli; Edema disease; F-18 FIMBRIAL ADHESIN; C-REACTIVE PROTEIN; HUMAN DENDRITIC CELLS; EDEMA DISEASE; B-SUBUNIT; MONOCLONAL-ANTIBODIES; POSTWEANING DIARRHEA; OXIDATIVE STRESS; TRANSGENIC MICE; F107; FIMBRIAE;

D O I：

10.1016/j.vaccine.2012.10.107

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Piglet edema disease is found worldwide and has historically been treated with antibiotics. However, no commercial vaccines are available for its prevention. In this study, the two major virulence factors of Shiga-toxigenic Escherichia coli (Stx2eB and FedF) were cloned to a pcDNA6.0 plasmid to develop a novel DNA vaccine against piglet edema disease. In animal trial in mouse model, the antibody titer, mortality, serum cytokine levels (interleukin-1 beta, interleukin-6, tumor necrosis factor alpha and C-reactive protein), serum malondialdehyde level and serum total superoxide dismutase activity were measured to validate the effectiveness of the DNA vaccine. The results show that Stx2eB and FedF at least partially protect against edema disease and FedF is more effective than Stx2eB. Co-immunization with both Stx2eB and FedF is most effective for protecting mice from a subsequent challenge with E. coli 0139 (which is known to cause edema disease in pigs). (C) 2012 Elsevier Ltd. All rights reserved.

引用

页码：367 / 372

页数：6

共 42 条

[1] EXPRESSION AND PURIFICATION OF SHIGA-LIKE TOXIN-II B-SUBUNITS [J].