Selective Lead Adsorption by Recombinant Escherichia coli Displaying a Lead-Binding Peptide

被引：28

作者：

Nguyen, Thuong T. L. ^{[1
]}

Lee, Hae Ryong ^{[1
]}

Hong, Soon Ho ^{[1
]}

Jang, Ji-Ryang ^{[2
]}

Choe, Woo-Seok ^{[2
,3
]}

Yoo, Ik-Keun ^{[1
]}

机构：

[1] Univ Ulsan, Sch Chem Engn & Bioengn, Ulsan 680749, South Korea

[2] Sungkyunkwan Univ, SKKU Adv Inst Nanotechnol SAINT, Suwon 440746, South Korea

[3] Sungkyunkwan Univ, Sch Chem Engn, Suwon 440746, South Korea

来源：

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY | 2013年 / 169卷 / 04期

关键词：

E; coli; Lead-binding peptide; Cell surface display; Biosorbent; Biosorption; SURFACE DISPLAY; HEAVY-METALS; BIOACCUMULATION; EXPRESSION; SEQUENCE; REMOVAL;

D O I：

10.1007/s12010-012-0073-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A highly specific lead-binding peptide ThrAsnThrLeuSerAsnAsn was displayed on Escherichia coli, and lead adsorption characteristics of the recombinant bacteria were investigated. Cell surface-displayed peptide was expressed under the control of an arabinose promoter using outer membrane protein C (OmpC(t)) as an anchoring motif. The optimal induction period and arabinose concentration for the expression of peptide-fused OmpC(t) were determined to be 2 h and 0.001 g/L, respectively. Selective adsorption of Pb2+ onto recombinant cells was verified with individual or combinatory use of four metal ions, Pb2+, Ni2+, Co2+, and Cu2+; the amount of bound Pb2+ onto the biosorbents was significantly higher than the other metal ions. The adsorption isotherm of recombinant cells for Pb2+ followed the Langmuir isotherm with a maximum adsorption loading (q (max)) of 526 mu mol/g dry cell weight.

引用

页码：1188 / 1196

页数：9

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