NHS-ester functionalized poly(PEGMA) brushes on silicon surface for covalent protein immobilization

被引：34
作者：
Yao, Yang [1 ]
Ma, Yong-Zheng [1 ]
Qin, Ming [1 ]
Ma, Xiao-Jing [2 ]
Wang, Chen [2 ]
Feng, Xi-Zeng [1 ]
机构：
[1] Nankai Univ, Key Lab Bioact Mat, Minist Educ, Coll Life Sci, Tianjin 300071, Peoples R China
[2] Natl Ctr Nanosci & Technol, Beijing 100080, Peoples R China
来源：
COLLOIDS AND SURFACES B-BIOINTERFACES | 2008年 / 66卷 / 02期
基金：
中国国家自然科学基金;
关键词：
poly(PEGMA); atom transfer radical polymerization (ATRP); NHS-ester functionalization; protein immobilization;
D O I：
10.1016/j.colsurfb.2008.06.015
中图分类号：
Q6 [生物物理学];
学科分类号：
071011 ;
摘要：
Poly(PEGMA) homopolymer brushes were developed by atom transfer radical polymerization (ATRP) on the initiator-modified Silicon Surface (Si-initiator). Through covalent binding, protein immobilization on the poly(PEGMA) films was enabled by further NHS-esterfunctionalization of the poly(PEGMA) chain ends. The formation of polymer brushes was confirmed by assessing the surface composition (XPS) and morphology (atomic force microscopy (AFM), scanning electronic microscopy (SEM)) of the modified silicon wafer. The binding performance of the NHS-ester functionalized surfaces with two Proteins horseradish peroxidase (HRP) and chicken immunoglobulin (IgG) was monitored by direct observation. These results suggest that this method which incorporates the properties of polymer brush onto the binding surfaces may be a good strategy Suitable for covalent Protein immobilization. (C) 2008 Elsevier B.V. All rights reserved.
引用
收藏
页码：233 / 239
页数：7
相关论文
共 25 条
  • [1] Toward optimized antibody microarrays:: a comparison of current microarray support materials
    Angenendt, P
    Glökler, J
    Murphy, D
    Lehrach, H
    Cahill, DJ
    [J]. ANALYTICAL BIOCHEMISTRY, 2002, 309 (02) : 253 - 260
  • [2] Immobilization of oriented protein molecules on poly(ethylene glycol)-coated Si(111)
    Cha, T
    Guo, A
    Jun, Y
    Pei, DQ
    Zhu, XY
    [J]. PROTEOMICS, 2004, 4 (07) : 1965 - 1976
  • [3] Polymer brushes via surface-initiated polymerizations
    Edmondson, S
    Osborne, VL
    Huck, WTS
    [J]. CHEMICAL SOCIETY REVIEWS, 2004, 33 (01) : 14 - 22
  • [4] Physicochemical properties of surface-initiated polymer films in the modification and processing of materials
    Jennings, GK
    Brantley, EL
    [J]. ADVANCED MATERIALS, 2004, 16 (22) : 1983 - 1994
  • [5] Amphiphilic polymer brushes grown from the silicon surface by atom transfer radical polymerization
    Kong, XX
    Kawai, T
    Abe, J
    Iyoda, T
    [J]. MACROMOLECULES, 2001, 34 (06) : 1837 - 1844
  • [6] Krieg R, 2003, CELL MOL BIOL, V49, P547
  • [7] Surface and interface control on photochemically initiated immobilization
    Liu, Li
    Engelhard, Mark H.
    Yan, Mingdi
    [J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2006, 128 (43) : 14067 - 14072
  • [8] Mercier A, 2001, MACROMOL CHEM PHYSIC, V202, P2672, DOI 10.1002/1521-3935(20010901)202:13<2672::AID-MACP2672>3.0.CO
  • [9] 2-A
  • [10] Minko S, 2001, MACROMOL RAPID COMM, V22, P206, DOI 10.1002/1521-3927(200102)22:3<206::AID-MARC206>3.0.CO
123