Ethylene glycol and glycolic acid production by wild-typeEscherichia coli

被引：16

作者：

Lu, Xiyang ^{[1
]}

Yao, Yao ^{[1
]}

Yang, Yang ^{[1
]}

Zhang, Zhongxi ^{[1
]}

Gu, Jinjie ^{[1
,4
]}

Mojovic, Ljiljana ^{[3
]}

Knezevic-Jugovic, Zorica ^{[3
]}

Baganz, Frank ^{[2
]}

Lye, Gary ^{[2
]}

Shi, Jiping ^{[1
,4
]}

Hao, Jian ^{[1
,2
]}

机构：

[1] Chinese Acad Sci, Shanghai Adv Res Inst, Lab Biorefinery, 99 Haike Rd, Shanghai 201210, Peoples R China

[2] UCL, Dept Biochem Engn, London, England

[3] Univ Belgrade, Fac Technol & Met, Belgrade, Serbia

[4] ShanghaiTech Univ, Schoo Life Sci & Technol, Shanghai, Peoples R China

来源：

BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY | 2021年 / 68卷 / 04期

基金：

上海市自然科学基金; 中国国家自然科学基金; 国家重点研发计划; 英国工程与自然科学研究理事会;

关键词：

E; coli; ethylene glycol; glycolic acid; xylonic acid; xylose; BIOTECHNOLOGICAL PRODUCTION; CORYNEBACTERIUM-GLUTAMICUM; ESCHERICHIA-COLI; GENE REPLACEMENT; XYLONIC ACID; BIOSYNTHESIS; PROTEIN; PATHWAY; XYLOSE;

D O I：

10.1002/bab.1987

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Ethylene glycol and glycolic acid are bulk chemicals with a broad range of applications. The ethylene glycol and glycolic acid biosynthesis pathways have been produced by microorganisms and used as a biological route for their production. Unlike the methods that use xylose or glucose as carbon sources, xylonic acid was used as a carbon source to produce ethylene glycol and glycolic acid in this study. Amounts of 4.2 g/L of ethylene glycol and 0.7 g/L of glycolic acid were produced by a wild-typeEscherichia coliW3110 within 10 H of cultivation with a substrate conversion ratio of 0.5 mol/mol. Furthermore,E. colistrains that produce solely ethylene glycol or glycolic acid were constructed. 10.3 g/L of glycolic acid was produced byE. coli Delta yqhD+aldA, and the achieved conversion ratio was 0.56 mol/mol. Similarly, theE. coli Delta aldA+yqhDproduced 8.0 g/L of ethylene glycol with a conversion ratio of 0.71 mol/mol. Ethylene glycol and glycolic acid production byE. colion xylonic acid as a carbon source provides new information on the biosynthesis pathway of these products and opens a novel way of biomass utilization.

引用

页码：744 / 755

页数：12

共 28 条

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