Chosen IL-17 family proteins in neutrophils of patients with oral inflammation

Purpose: Recent studies have indicated that human neutrophils (PMN), belonging to the first line of cellular defence against various infectious agents and inflammation can produce cytokines of the interleukin 17 (IL-17) family. The relation between pro-inflammatory IL-17A and anti-inflammatory IL-17E may be responsible for the development and maintenance of the inflammatory process. The study objective was to determine the expression of IL-17E and IL-17BR in confrontation with IL-17A and IL-17R in neutrophils of patients with oral cavity inflammation and to compare protein expressions in autologous peripheral blood mononuclear cells (PBMC). Material and Methods: Expressions of IL-17E, IL-17A, IL-17BR and IL-17R was assessed by the Western blot method. IL-17E and IL-17A levels was determined by the ELISA method. Results: The expressions of IL-17E, IL-17A, IL-17BR and IL-17R in PMN and PBMC in patients were higher in the cells of patients, as compared to the controls. The expressions of IL-17E and IL-17BR in both kinds of cells were lower than the levels of IL-17A and IL-17R. The levels of IL-17E and IL-17A were increased in cell supernatants and blood serum of patients, as compared to the control group. Conclusions: The preliminary examinations indicate the predominance of pro-inflammatory effects of IL-17A controlled by PMN, as well as PBMC in patients with oral inflammation. Results obtained also suggest a more significant involvement of PMN in the IL-17E and IL-17A dependent reactions.