A Protein-Interaction Array Inside a Living Cell

被引：38

作者：

Gandor, Silke ^{[1
,2
]}

Reisewitz, Stephanie ^{[2
]}

Venkatachalapathy, Muthukumaran ^{[1
,2
]}

Arrabito, Giuseppe ^{[2
,3
]}

Reibner, Martina ^{[2
]}

Schroeder, Hendrik ^{[2
]}

Ruf, Katharina ^{[1
,2
]}

Niemeyer, Christof M. ^{[2
,4
]}

Bastiaens, Philippe I. H. ^{[1
,2
]}

Dehmelt, Leif ^{[1
,2
]}

机构：

[1] Max Planck Inst Mol Physiol, Dept Syst Cell Biol, D-44227 Dortmund, Germany

[2] Tech Univ Dortmund, D-44227 Dortmund, Germany

[3] Scuola Super Catania, I-95123 Catania, Italy

[4] KIT, Inst Biol Interfaces IBG 1, D-76344 Eggenstein Leopoldshafen, Germany

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2013年 / 52卷 / 18期

关键词：

multiplexed assay; protein-protein interactions; receptors; signal transduction; surface-immobilization; SITE MUTATIONS DEFINE;

D O I：

10.1002/anie.201209127

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Taking the bait: Protein-interaction arrays were generated in living cells by the interaction of bait-presenting artificial receptor constructs (bait-PARCs) with micrometer-scaled antibody surface patterns (see figure). This method was applied to simultaneously monitor the interaction kinetics of a prey protein with two distinct bait proteins in individual living cells. Copyright © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

引用

页码：4790 / 4794

页数：5

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