A sensitive and specific method for microRNA detection andin situimaging based on a CRISPR-Cas9 modified catalytic hairpin assembly

被引：25

作者：

Liu, Yang ^{[1
]}

Li, Shihong ^{[1
]}

Zhang, Likun ^{[1
]}

Zhao, Qian ^{[1
]}

Li, Nuo ^{[1
]}

Wu, Yuxin ^{[1
]}

机构：

[1] Jinzhou Med Univ, Dept Clin Lab, Affiliated Hosp 3, Jinzhou 121000, Liaoning, Peoples R China

来源：

RSC ADVANCES | 2020年 / 10卷 / 47期

关键词：

MIRNA;

D O I：

10.1039/d0ra03603j

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

We report here a method for the molecular detection of miRNAs in exosomes and imaging in living cells based on CRISPR-Cas9 and catalytic hairpin assembly. With the cascade signal amplification of catalytic hairpin assembly and high specific recognition of CRISPR-Cas9, the detection method showed a sensitivity of 23.5 fM and was applied for accurate exosomal miR-21 detection andin situmiR-21 imaging.

引用

页码：28037 / 28040

页数：4

共 25 条

[21] Detection of target DNA with a novel Cas9/sgRNAs-associated reverse PCR (CARP) technique
Zhang, Beibei
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[22] A Dual-Signal Twinkling Probe for Fluorescence-SERS Dual Spectrum Imaging and Detection of miRNA in Single Living Cell via Absolute Value Coupling of Reciprocal Signals
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[J]. ACS SENSORS, 2019, 4 (04): : 924 - +
[23] Allosteric Probe-Initiated Wash-Free Method for Sensitive Extracellular Vesicle Detection through Dual Cycle-Assisted CRISPR-Cas12a
Zhao, Xianxian
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[J]. ACS SENSORS, 2020, 5 (07) : 2239 - 2246
[24] Aptamer-Cholesterol-Mediated Proximity Ligation Assay for Accurate Identification of Exosomes
Zhao, Xianxian
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[J]. ANALYTICAL CHEMISTRY, 2020, 92 (07) : 5411 - 5418
[25] Rapid and sensitive exosome detection with CRISPR/Cas12a
Zhao, Xianxian
Zhang, Wenqing
Qiu, Xiaopei
Mei, Qiang
Luo, Yang
Fu, Weiling
[J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2020, 412 (03) : 601 - 609

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