Gold-nanoparticle-based biosensors for detection of enzyme activity

被引:124
作者
Hutter, Eliza [1 ]
Maysinger, Dusica [1 ]
机构
[1] McGill Univ, Dept Pharmacol & Therapeut, Montreal, PQ H3G 1Y6, Canada
基金
加拿大自然科学与工程研究理事会; 加拿大健康研究院;
关键词
colorimetric assays; FRET; transferases; hydrolases; matrix metalloproteinases; PROTEIN-KINASE ACTIVITY; MATRIX-METALLOPROTEINASE ACTIVITY; COLORIMETRIC ASSAY; ELECTROGENERATED CHEMILUMINESCENCE; SIGNAL AMPLIFICATION; CYTOCHROME-C; LIVE CELLS; INHIBITION; AGGREGATION; PROBES;
D O I
10.1016/j.tips.2013.07.002
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
Optimal enzyme activity is essential for maintenance of physiological homeostasis. A variety of both non-genetic and genetic disruptions can excessively activate or silence intrinsic enzyme activities, with pathological outcomes. Many pharmacological agents are activators and inhibitors of enzymes. It is essential, therefore, in the development of drugs as enzyme activators and inhibitors, that enzyme activities be accurately measured under physiological and pathological conditions. Different biochemical assays have been developed for this purpose, some of which are based on nanostructured materials. This review focuses on gold nanoparticle (GNP)-based structures for the sensing and measurement of enzyme activities in biological specimens. Here we provide an overview and critical analysis of such assays, identify their advantages and limitations, and discuss interesting features of GNPs to be exploited for future applications in pharmacology.
引用
收藏
页码:497 / 507
页数:11
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