Isolation and characterization of collagen from the skin of Brama australis

被引：41
|
作者：
Sionkowska, Alina [1 ]
Kozlowska, Justyna [1 ]
Skorupska, Malgorzata [1 ]
Michalska, Marta [1 ]
机构：
[1] Nicolaus Copernicus Univ, Fac Chem, Dept Chem Biomat & Cosmet, PL-87100 Torun, Poland
来源：
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | 2015年 / 80卷
关键词：
Collagen; Fish skin; Denaturation temperature; ACID-SOLUBLE COLLAGEN; FISH; PROTEINS; SCALE;
D O I：
10.1016/j.ijbiomac.2015.07.032
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Collagen was extracted from the skin of Brama australis, the fish from warm-water sea. The yield of collagen from skin of B. australis was about 1.5% on a wet weight basis of raw material. The isolated protein was confirmed as collagen by different physico-chemical techniques such as: FTIR, SOS-PAGE, and amino acid analysis. The denaturation temperature (T-d) of obtained collagen was found to be 24 degrees C, what is promising as an advantage for cosmetic application. According to the electrophoretic pattern, collagen consisted of two different alpha-chains (alpha 1 and alpha 2) and was classified as type I collagen. Although T-d of obtained collagen is higher than 20 degrees C it is still far from T-d of mammalian collagen. (C) 2015 Elsevier B.V. All rights reserved.
引用
收藏
页码：605 / 609
页数：5
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