The 2b protein and the C-terminus of the 2a protein of cucumber mosaic virus subgroup I strains both play a role in viral RNA accumulation and induction of symptoms

被引：39
作者：
Du, Zhiyou [1 ]
Chen, Feifei [1 ]
Zhao, Zhijing [1 ]
Liao, Qiansheng [1 ]
Palukaitis, Peter [2 ]
Chen, Jishuang [1 ]
机构：
[1] Zhejiang Sci Tech Univ, Inst Bioengn, Hangzhou 310018, Zhejiang, Peoples R China
[2] Scottish Crop Res Inst, Dundee DD2 5DA, Scotland
来源：
VIROLOGY | 2008年 / 380卷 / 02期
基金：
中国国家自然科学基金;
关键词：
Cucumber mosaic virus; Chimeric virus; 2a protein; 2b protein; Systemic necrosis; Pathogenicity;
D O I：
10.1016/j.virol.2008.07.036
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Two chimeras of cucumber mosaic virus (CMV), FCb7(2b)-CMV and FRad35(2b)-CMV, with the 2b genes of strains Cb7-CMV and Rad35-CMV, respectively, in an Fny-CMV background, gave different responses on Nicotiana glutinosa: FCb7(2b)-CMV induced systemic necrosis while FRad35(2b)-CMV caused only mild mosaic. This differential virulence was attributable to the nature of amino acid 55 of their 2b proteins, However, sequence analysis revealed that Leu(55) of the 2b Protein was necessary but not sufficient for FCb7(2b)-CMV to induce systemic necrosis. Surprisingly, inhibition of translation of the 2a/2b overlapping region of the 2a protein in FCb7(2b)-CMV led to a loss of systemic necrosis and a reduction in accumulation of viral progeny RNAs. The 2a/2b overlapping region of Fny-CMV had a Similar effect on virulence and vital accumulation. Thus, the 2a protein C-terminus of subgroup I strains, as well as the 2b protein, play a role in symptom induction and accumulation of viral RNAs. (C) 2008 Elsevier Inc. All rights reserved.
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收藏
页码：363 / 370
页数：8
相关论文
共 31 条
[1]   RETRACTED: Viral pathogenicity determinants are suppressors of transgene silencing in Nicotiana benthamiana (Retracted article. See vol. 34, pg. 2595, 2015) [J].
Brigneti, G ;
Voinnet, O ;
Li, WX ;
Ji, LH ;
Ding, SW ;
Baulcombe, DC .
EMBO JOURNAL, 1998, 17 (22) :6739-6746
[2]   Viral RNA silencing suppressors inhibit the microRNA pathway at an intermediate step [J].
Chapman, EJ ;
Prokhnevsky, AI ;
Gopinath, K ;
Dolja, VV ;
Carrington, JC .
GENES & DEVELOPMENT, 2004, 18 (10) :1179-1186
[3]   LONG-DISTANCE MOVEMENT FACTOR - A TRANSPORT FUNCTION OF THE POTYVIRUS HELPER COMPONENT PROTEINASE [J].
CRONIN, S ;
VERCHOT, J ;
HALDEMANCAHILL, R ;
SCHAAD, MC ;
CARRINGTON, JC .
PLANT CELL, 1995, 7 (05) :549-559
[4]   Suppression of antiviral silencing by cucumber mosaic virus 2b protein in Arabidopsis is associated with drastically reduced accumulation of three classes of viral small interfering RNAs [J].
Diaz-Pendon, Juan A. ;
Li, Feng ;
Li, Wan-Xiang ;
Ding, Shou-Wei .
PLANT CELL, 2007, 19 (06) :2053-2063
[5]   An interspecies hybrid RNA virus is significantly more virulent than either parental virus [J].
Ding, SW ;
Shi, BJ ;
Li, WX ;
Symons, RH .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1996, 93 (15) :7470-7474
[6]   A NOVEL NATURALLY-OCCURRING HYBRID GENE ENCODED BY A PLANT RNA VIRUS FACILITATES LONG-DISTANCE VIRUS MOVEMENT [J].
DING, SW ;
LI, WX ;
SYMONS, RH .
EMBO JOURNAL, 1995, 14 (23) :5762-5772
[7]   NEW OVERLAPPING GENE ENCODED BY THE CUCUMBER MOSAIC-VIRUS GENOME [J].
DING, SW ;
ANDERSON, BJ ;
HAASE, HR ;
SYMONS, RH .
VIROLOGY, 1994, 198 (02) :593-601
[8]   The necrotic pathotype of the Cucumber mosaic virus (CMV) Ns strain is solely determined by amino acid 461 of the 1a protein [J].
Divéki, Z ;
Salánki, K ;
Balázs, E .
MOLECULAR PLANT-MICROBE INTERACTIONS, 2004, 17 (08) :837-845
[9]   2b ORFs encoded by subgroup IB strains of cucumber mosaic virus induce differential virulence on Nicotiana species [J].
Du, Zhi-You ;
Chen, Fei-Fei ;
Liao, Oian-Sheng ;
Zhang, Hua-Rong ;
Cheni, Yan-Fei ;
Chen, Ji-Shuang .
JOURNAL OF GENERAL VIROLOGY, 2007, 88 :2596-2604
[10]   Characterization of silencing suppressor 2b of cucumber mosaic virus based on examination of its small RNA-Binding abilities [J].
Goto, Kazunori ;
Kobori, Takashi ;
Kosaka, Yoshitaka ;
Natsuaki, Tomohide ;
Masuta, Chikara .
PLANT AND CELL PHYSIOLOGY, 2007, 48 (07) :1050-1060
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