Biosynthesis and Characterization of Poly-(3-hydroxybutyrate-co-3-hydroxyvalerate) from Bacillus cereus S10

被引：19

作者：

Masood, Farha ^{[1
]}

Hasan, Fariha ^{[1
]}

Ahmed, Safia ^{[1
]}

Chen, P. ^{[2
]}

Hameed, Abdul ^{[1
]}

机构：

[1] Quaid I Azam Univ, Dept Microbiol, Islamabad, Pakistan

[2] Univ Waterloo, Dept Chem Engn, Waterloo, ON N2L 3G1, Canada

来源：

JOURNAL OF POLYMERS AND THE ENVIRONMENT | 2012年 / 20卷 / 03期

关键词：

Biodegradable PHA copolymer; Biocompatible; Poly-(3HB-co-5 mol% 3HV); Bacillus cereus; Nanoparticles; BACTERIAL SYNTHESIS; POLYHYDROXYALKANOATES; MEGATERIUM; PHB; POLYHYDROXYBUTYRATE;

D O I：

10.1007/s10924-012-0457-y

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

The biodegradable and biocompatible copolymer poly-(3-hydroxybutyrate-co-5 mol% 3-hydroxyvalerate), poly-(3HB-co-5 mol% 3HV), was synthesized by Bacillus cereus S10 and the highest yield was determined as 69.91 % at pH 7 and 30 A degrees C after 48 h of incubation using a glucose as the sole carbon source. Poly-(3HB-co-5 mol% 3HV) was purified from bacterial biomass using chloroform. FTIR analysis showed absorption bands at 1,723, 1,274, 1,373, 1,453, 2,932 cm(-1) corresponding to C=O, C-O stretching, CH3, -CH2 and -CH groups, respectively. H-1-NMR and C-13-NMR analysis confirmed that the copolymer was composed of 95 mol% of 3-hydroxybutrate and 5 mol% of 3-HV monomeric units. Poly-(3-HB-co-5 mol% 3HV) was used for nanoparticles preparation. The diameter of nanoparticles was 202 nm.

引用

页码：865 / 871

页数：7

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