Biochemical Characterization of Novel Bioactive Protein from Silkworm (Bombyx mori L) Fecal Matter

被引：1

作者：

Raghavendra, R. ^{[1
]}

Neelagund, S. E. ^{[1
]}

机构：

[1] Kuvempu Univ, Dept Post Grad Studies & Res Biochem, Shivamogga 577451, Karnataka, India

来源：

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY | 2012年 / 167卷 / 05期

关键词：

Novel protein; Purification; Characterization; Bombyx mori; ALDI-TOF-MS; LC-ESI-MS/MS; INDUCIBLE ANTIBACTERIAL PEPTIDES; INSECT IMMUNITY; COLEOPTERAN INSECT; PURIFICATION; LARVAE; HEMOLYMPH; FAMILY; PEPTIDOGLYCAN; DIPTERICIN; EXPRESSION;

D O I：

10.1007/s12010-012-9588-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In this study, complete purification and biochemical characterization of protein is presented. The protein was purified by using Sephadex G-75 gel filtration column followed by reverse-phase high-performance liquid chromatography in a C18 column. The molecular weight of the protein was determined by sodium dodecyl sulfate polyacrylamide gel electrophoresis, mass spectrum matrix-assisted laser desorption/ionization-time-of-flight-mass spectrometry (MALDI-TOF-MS) and liquid chromatography-electrospray ionization tandem mass spectrometry. Protein was fragmented by trypsin based on the m/z values obtained by MALDI-TOF-MS analysis. The peptide fragments sequence showed homology with DEAD-box-ATP-dependent RNA helicase 45, present in a public domain, National Centre for Biotechnology Information. The protein exhibited antibacterial activity against selected Gram +/- bacteria. The analgesic activity was determined by conducting acetic-acid-induced writhing test in mice.

引用

页码：1002 / 1014

页数：13

共 39 条

[31]

Raghavendra R, 2009, INT J BIOMED PHARM S, V3, P74

[32] Protective effect of partially purified 35 kDa protein from silk worm (Bombyx mori) fecal matter against carbon tetrachloride induced hepatotoxicity and in vitro anti-viral properties [J].

Raghavendra, Ramappa ;

Neelagund, Shivayogeeswar ;

Kuluvar, Gouthamchandra ;

Bhanuprakash, Veerakyathappa ;

Revanaiah, Yogisharadhya .

PHARMACEUTICAL BIOLOGY, 2010, 48 (12) :1426-1431

[33] Purification, characterization and gene expression of a glycine and proline-rich antibacterial protein family from larvae of a beetle, Allomyrina dichotoma [J].

Sagisaka, A ;

Miyanoshita, A ;

Ishibashi, J ;

Yamakawa, M .

INSECT MOLECULAR BIOLOGY, 2001, 10 (04) :293-302

[34]

Satyanarayana K., 1993, Indian Journal of Pharmaceutical Sciences, V55, P230

[35]

Sharath R., 2008, PHARMACOLOGYONLINE, V2, P517

[36]

Sharma J, 2005, CURR SCI INDIA, V89, P1613

[37] INDUCTION OF ANTIBACTERIAL ACTIVITY IN THE HEMOLYMPH OF THE SILKWORM, BOMBYX-MORI, BY INJECTION OF FORMALIN-TREATED ESCHERICHIA-COLI K-12 IN THE ANTERIOR AND POSTERIOR BODY PART OF THE LIGATED LARVAE [J].

SUMIDA, M ;

ICHIMORI, H ;

YUHKI, T ;

MORI, H ;

MATSUBARA, F .

COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY, 1992, 101 (1-2) :173-178

[38] A VIRUS-INACTIVATING PROTEIN ISOLATED FROM THE DIGESTIVE JUICE OF THE SILKWORM, BOMBYX-MORI [J].

UCHIDA, Y ;

KAWAMOTO, F ;

HIMENO, M ;

HAYASHIYA, K .

JOURNAL OF INVERTEBRATE PATHOLOGY, 1984, 43 (02) :182-189

[39]

WICKER C, 1990, J BIOL CHEM, V265, P22493

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