Biochemical Characterization of Novel Bioactive Protein from Silkworm (Bombyx mori L) Fecal Matter

被引：1

作者：

Raghavendra, R. ^{[1
]}

Neelagund, S. E. ^{[1
]}

机构：

[1] Kuvempu Univ, Dept Post Grad Studies & Res Biochem, Shivamogga 577451, Karnataka, India

来源：

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY | 2012年 / 167卷 / 05期

关键词：

Novel protein; Purification; Characterization; Bombyx mori; ALDI-TOF-MS; LC-ESI-MS/MS; INDUCIBLE ANTIBACTERIAL PEPTIDES; INSECT IMMUNITY; COLEOPTERAN INSECT; PURIFICATION; LARVAE; HEMOLYMPH; FAMILY; PEPTIDOGLYCAN; DIPTERICIN; EXPRESSION;

D O I：

10.1007/s12010-012-9588-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In this study, complete purification and biochemical characterization of protein is presented. The protein was purified by using Sephadex G-75 gel filtration column followed by reverse-phase high-performance liquid chromatography in a C18 column. The molecular weight of the protein was determined by sodium dodecyl sulfate polyacrylamide gel electrophoresis, mass spectrum matrix-assisted laser desorption/ionization-time-of-flight-mass spectrometry (MALDI-TOF-MS) and liquid chromatography-electrospray ionization tandem mass spectrometry. Protein was fragmented by trypsin based on the m/z values obtained by MALDI-TOF-MS analysis. The peptide fragments sequence showed homology with DEAD-box-ATP-dependent RNA helicase 45, present in a public domain, National Centre for Biotechnology Information. The protein exhibited antibacterial activity against selected Gram +/- bacteria. The analgesic activity was determined by conducting acetic-acid-induced writhing test in mice.

引用

页码：1002 / 1014

页数：13

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