Bioactive glass modulation of intestinal epithelial cell restitution

被引：28
作者：
Moosvi, Syed Raza [1 ]
Day, Richard M. [1 ]
机构：
[1] UCL, Ctr Gastroenterol & Nutr, Windeyer Inst, Biomat & Tissue Engn Grp, London W1T 4JF, England
来源：
ACTA BIOMATERIALIA | 2009年 / 5卷 / 01期
关键词：
Bioactive glass; Epithelial; Restitution; ANGIOGENIC GROWTH-FACTORS; IN-VITRO; FACTOR-BETA; MONOLAYERS; MIGRATION; RELEASE; REPAIR;
D O I：
10.1016/j.actbio.2008.08.003
中图分类号：
R318 [生物医学工程];
学科分类号：
0831 ;
摘要：
Repair of superficial injury to the gastrointestinal mucosa involves the process of restitution, the rapid migration of epithelial cells across damaged areas. The effect of 45S5 bioactive glass on epithelial restitution was assessed using a novel co-culture model incorporating wounded intestinal epithelial cell monolayers and sub-epithelial myofibroblasts to simulate in vivo conditions that occur during superficial mucosal ulceration. Epithelial wound healing was not increased by culture medium conditioned with bioactive glass, with 1% (w/v) bioactive glass inhibiting cell migration. Epithelial wounds co-cultured with myofibroblasts grown on surfaces coated with 0.1% (w/v) bioactive glass increased wound healing compared with co-cultures containing no bioactive glass. Myofibroblasts grown on surfaces coated with bioactive glass secreted significantly increased amounts of basic fibroblast growth factor but did not increase epithelial cell proliferation, indicating the wound healing observed was due to restitution. These data from a model of superficial mucosal injury suggest bioactive glass may function as a stimulant of paracrine mucosal signaling networks that promotes rapid epithelial repair. (C) 2008 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.
引用
收藏
页码：76 / 83
页数：8
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