Potential biomarkers for antidiastole of tuberculous and malignant pleural effusion by proteome analysis

被引：7

作者：

Shi, Jing ^{[1
]}

Li, Pu ^{[2
]}

Zhou, Lijin ^{[2
]}

Qi, Suwen ^{[3
]}

Wang, Bo ^{[2
]}

Li, Dandan ^{[2
]}

Duan, Liang ^{[2
]}

Chen, Wei Xian ^{[2
]}

Xia, Jirong ^{[1
]}

Zou, Lin ^{[1
]}

Yang, Shuangshuang ^{[1
]}

机构：

[1] Chongqing Med Univ, Affiliated Hosp 1, Dept Lab Med, Chongqing 400016, Peoples R China

[2] Chongqing Med Univ, Affiliated Hosp 2, Dept Lab Med, Chongqing 400010, Peoples R China

[3] Shenzhen Univ, Natl Reg Key Technol Engn Lab Med Ultrasound, Guangdong Key Lab Biomed Measurements & Ultrasoun, Dept Biomed Engn,Sch Med, Shenzhen 518060, Peoples R China

来源：

BIOMARKERS IN MEDICINE | 2019年 / 13卷 / 02期

基金：

美国国家科学基金会;

关键词：

cathepsin G; fibronectin; iTRAQTM; leukotriene-A4; hydrolase; lung cancer; malignant pleural effusion; potential biomarkers; quantitative proteomic analyze; tuberculosis; tuberculous pleural effusion; ADENOSINE-DEAMINASE; DIAGNOSTIC YIELD; CATHEPSIN-G; FIBRONECTIN; MANAGEMENT; EXPRESSION; SURVIVAL;

D O I：

10.2217/bmm-2018-0200

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

Aim: To investigate novel potential biomarkers for antidiastole of tuberculous pleural effusion (TPE) from malignant pleural effusion (MPE). Materials & methods: iTRAQTM-coupled LC-MS/MS were applied to analyze the proteome of TPE and MPE samples. The candidate proteins were verified by enzyme-linked immunosorbent assay. Results: A total of 432 differential proteins were identified. Enzyme-linked immunosorbent assay revealed significantly higher levels of fibronectin (FN) and cathepsin G (CTSG) in MPE than in TPE, but lower levels of leukotriene-A4 hydrolase (LTA4H). The receiver operator characteristic values were 0.285 for FN, 0.64 for LTA4H, 0.337 for CTSG and 0.793 for a combination of these candidate markers. Conclusion: FN, LTA4H and CTSG were identified as potential biomarkers to differentiate TPE from MPE and their combination exhibited higher diagnostic capacity.

引用

页码：123 / 133

页数：11

共 43 条

[1] Effect of the leukotriene A4 hydrolase aminopeptidase augmentor 4-methoxydiphenylmethane in a pre-clinical model of pulmonary emphysema [J].

De Oliveira, Eliseu O. ;

Wang, Kan ;

Kong, Hye-Sik ;

Kim, Suhyon ;

Miessau, Matthew ;

Snelgrove, Robert J. ;

Shim, Y. Michael ;

Paige, Mikell .

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, 2011, 21 (22) :6746-6750

[2] Utility of quantitative T-cell responses versus unstimulated interferon-γ for the diagnosis of pleural tuberculosis [J].

Dheda, K. ;

van Zyl-Smit, R. N. ;

Sechi, L. A. ;

Badri, M. ;

Meldau, R. ;

Meldau, S. ;

Symons, G. ;

Semple, P. L. ;

Maredza, A. ;

Dawson, R. ;

Wainwright, H. ;

Whitelaw, A. ;

Vallie, Y. ;

Raubenheimer, P. ;

Bateman, E. D. ;

Zumla, A. .

EUROPEAN RESPIRATORY JOURNAL, 2009, 34 (05) :1118-1126

[3] Diagnostic tools in tuberculous pleurisy: a direct comparative study [J].

Diacon, AH ;

Van de Wal, BW ;

Wyser, C ;

Smedema, JP ;

Bezuidenhout, J ;

Bolliger, CT ;

Walzl, G .

EUROPEAN RESPIRATORY JOURNAL, 2003, 22 (04) :589-591

[4] iTRAQ-based quantitative proteomic analysis of cervical cancer [J].

Ding, Yibing ;

Yang, Min ;

She, Sha ;

Min, Haiyan ;

Xv, Xiaoming ;

Ran, Xiaoping ;

Wu, Yongzheng ;

Wang, We ;

Wang, Lei ;

Yi, Long ;

Yang, Yixuan ;

Gao, Qian .

INTERNATIONAL JOURNAL OF ONCOLOGY, 2015, 46 (04) :1748-1758

[5] Cystatin C of pleural effusion as a novel diagnostic aid in pleural diseases of different aetiologies [J].

Domej, W ;

Tilz, GP ;

Földes-Papp, Z ;

Demel, U ;

Rabold, T ;

Holzer, H .

CLINICAL SCIENCE, 2002, 102 (03) :373-380

[6] Tuberculous Pleural Effusion [J].

Ferreiro, Lucia ;

San Jose, Esther ;

Valdes, Luis .

ARCHIVOS DE BRONCONEUMOLOGIA, 2014, 50 (10) :435-443

[7] Diagnosis and treatment of tuberculous pleural effusion in 2006 [J].

Gopi, Arun ;

Madhavan, Sethu M. ;

Sharma, Surendra K. ;

Sahn, Steven A. .

CHEST, 2007, 131 (03) :880-889

[8] Neutrophilic Cathepsin C Is Maturated by a Multistep Proteolytic Process and Secreted by Activated Cells during Inflammatory Lung Diseases [J].

Hamon, Yveline ;

Legowska, Monika ;

Herve, Virginie ;

Dallet-Choisy, Sandrine ;

Marchand-Adam, Sylvain ;

Vanderlynden, Lise ;

Demonte, Michele ;

Williams, Rich ;

Scott, Christopher J. ;

Si-Tahar, Mustapha ;

Heuze-Vourc'h, Nathalie ;

Lalmanach, Gilles ;

Jenne, Dieter E. ;

Lesner, Adam ;

Gauthier, Francis ;

Korkmaz, Brice .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2016, 291 (16) :8486-8499

[9] A METHOD OF COMPARING THE AREAS UNDER RECEIVER OPERATING CHARACTERISTIC CURVES DERIVED FROM THE SAME CASES [J].

HANLEY, JA ;

MCNEIL, BJ .

RADIOLOGY, 1983, 148 (03) :839-843

[10] Recent advances in the diagnosis and management of malignant pleural effusions [J].

Heffner, John E. ;

Klein, Jeffrey S. .

MAYO CLINIC PROCEEDINGS, 2008, 83 (02) :235-250

← 1 2 3 4 5 →