Diels Alder reaction strategy to synthesize 1,2,3,6-tetrahydro-1,2,4,5-tetrazines and exploration of their anti-inflammatory potential

The aim of this study was to investigate the anti-inflammatory potential elicited by 1,2,3,6tetrahydro-1,2,4,5-tetrazines. It was performed on albino mice after syringe administration (500 mg/Kg, 1000 mg/Kg, 1500 mg/Kg) using carrgeenan induced paw edema as an acute inflammatory model. In this test 4-(2,3,6-triphenyl-3,6-dihydro-1,2,4,5-tetrazin-1(2H)-yl)phenol 7a and 4-[3,6-diphenyl-2-(4-nitrophenyl)-3,6-dihydro-1,2,4,5-tetrazin-1(2H)-yl]phenol 7b significantly impaired both early and late phases of the inflammatory responses. All the tested 1,2,3,6-tetrahydro-1,2,4,5-tetrazines were synthesized and their structures were corroborated by spectroanalytical analysis (IR, H-1 NMR, C-13 NMR, Mass spectrometry).