Bio-Inspired NanoVilli Chips for Enhanced Capture of Tumor-Derived Extracellular Vesicles: Toward Non-Invasive Detection of Gene Alterations in Non-Small Cell Lung Cancer

被引：68

作者：

Dong, Jiantong ^{[1
,2
]}

Zhang, Ryan Y. ^{[2
]}

Sun, Na ^{[2
,9
]}

Smalley, Matthew ^{[2
]}

Wu, Zipeng ^{[2
]}

Zhou, Anqi ^{[2
]}

Chou, Shih-Jie ^{[2
,10
]}

Jan, Yu Jen ^{[2
,11
]}

Yang, Peng ^{[2
]}

Bao, Lirong ^{[2
]}

Qi, Dongping ^{[2
]}

Tang, Xinghong ^{[2
]}

Tseng, Patrick ^{[2
]}

Hua, Yue ^{[2
]}

Xu, Dianwen ^{[2
]}

Kao, Rueihung ^{[2
]}

Meng, Meng ^{[2
,12
,13
]}

Zheng, Xirun ^{[14
]}

Liu, Ying ^{[14
]}

Vagner, Tatyana ^{[15
,16
,17
]}

Chai, Xiaoshu ^{[14
]}

Zhou, Dongjing ^{[14
]}

Li, Mengyuan ^{[1
]}

Chiou, Shih-Hwa ^{[10
]}

Zheng, Guangjuan ^{[14
]}

Di Vizio, Dolores ^{[15
,16
,17
]}

Agopian, Vatche G. ^{[3
]}

Posadas, Edwin ^{[11
]}

Jonas, Steven J. ^{[4
,5
,6
,7
,8
]}

Ju, Shin-Pon ^{[18
]}

Weiss, Paul S. ^{[6
,7
,8
]}

Zhao, Meiping ^{[1
]}

Tseng, Hsian-Rong ^{[2
]}

Zhu, Yazhen ^{[2
,14
]}

机构：

[1] Peking Univ, Beijing Natl Lab Mol Sci, Coll Chem & Mol Engn, MOE Key Lab Bioorgan Chem & Mol Engn, Beijing 100871, Peoples R China

[2] Univ Calif Los Angeles, Calif NanoSyst Inst, Crump Inst Mol Imaging, Dept Mol & Med Pharmacol, Los Angeles, CA 90095 USA

[3] Univ Calif Los Angeles, Dept Surg, Los Angeles, CA 90095 USA

[4] Univ Calif Los Angeles, David Geffen Sch Med, Dept Pediat, Eli & Edythe Broad Ctr Regenerat Med & Stem Cell, Los Angeles, CA 90095 USA

[5] Univ Calif Los Angeles, Childrens Discovery & Innovat Inst, Los Angeles, CA 90095 USA

[6] Univ Calif Los Angeles, Calif NanoSyst Inst, Los Angeles, CA 90095 USA

[7] Univ Calif Los Angeles, Dept Chem & Biochem, Los Angeles, CA 90095 USA

[8] Univ Calif Los Angeles, Dept Mat Sci & Engn, Los Angeles, CA 90095 USA

[9] Chinese Acad Sci, Suzhou Inst Nanotech & Nanobion, Key Lab Nanobio Interface, Suzhou 215123, Peoples R China

[10] Natl Yang Ming Univ, Inst Pharmacol, Taipei 112, Taiwan

[11] Cedars Sinai Med Ctr, Samuel Oschin Comprehens Canc Inst, Los Angeles, CA 90048 USA

[12] Nankai Univ, Coll Pharm, State Key Lab Med Chem Biol, Haihe Educ Pk,38 Tongyan Rd, Tianjin 300353, Peoples R China

[13] Nankai Univ, Tianjin Key Lab Mol Drug Res, Haihe Educ Pk,38 Tongyan Rd, Tianjin 300353, Peoples R China

[14] Guangzhou Univ Chinese Med, Guangdong Prov Acad Chinese Med Sci, Guangdong Prov Hosp Tradit Chinese Med, Dept Pathol, Guangzhou 510120, Guangdong, Peoples R China

[15] Samuel Oschin Comprehens Canc Inst, Div Canc Biol & Therapeut, Dept Surg, Los Angeles, CA 90048 USA

[16] Samuel Oschin Comprehens Canc Inst, Div Canc Biol & Therapeut, Dept Biomed Sci, Los Angeles, CA 90048 USA

[17] Samuel Oschin Comprehens Canc Inst, Div Canc Biol & Therapeut, Dept Pathol & Lab Med, Los Angeles, CA 90048 USA

[18] Natl Sun Yat Sen Univ, Dept Mech & Electromech Engn, Kaohsiung 80424, Taiwan

来源：

ACS APPLIED MATERIALS & INTERFACES | 2019年 / 11卷 / 15期

基金：

美国国家卫生研究院;

关键词：

nanosubstrates; microfluidics; extracellular vesicles; ROS1; rearrangements; EGFR T790M mutation; non-small cell lung cancer; EXOSOMES; MICROVESICLES; FILTRATION; SEPARATION; MICRORNA; ARRAYS;

D O I：

10.1021/acsami.9b01406

中图分类号：

TB3 [工程材料学];

学科分类号：

0805 ; 080502 ;

摘要：

Tumor-derived extracellular vesicles (EVs) present in bodily fluids are emerging liquid biopsy markers for non-invasive cancer diagnosis and treatment monitoring. Because the majority of EVs in circulation are not of tumor origin, it is critical to develop new platforms capable of enriching tumor-derived EVs from the blood. Herein, we introduce a biostructure-inspired NanoVilli Chip, capable of highly efficient and reproducible immunoaffinity capture of tumor-derived EVs from blood plasma samples. Anti-EpCAMgrafted silicon nanowire arrays were engineered to mimic the distinctive structures of intestinal microvilli, dramatically increasing surface area and enhancing tumor-derived EV capture. RNA in the captured EVs can be recovered for downstream molecular analyses by reverse transcription Droplet Digital PCR. We demonstrate that this assay can be applied to monitor the dynamic changes of ROS1 rearrangements and epidermal growth factor receptor T790M mutations that predict treatment responses and disease progression in non -small cell lung cancer patients.

引用

页码：13973 / 13983

页数：11

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