Quantitative determination of indole-3-acetic acid in yeasts using high performance liquid chromatography-tandem mass spectrometry

被引：16
作者：
Streletskii, R. A. [1 ]
Kachalkin, A. V. [1 ]
Glushakova, A. M. [1 ]
Demin, V. V. [1 ]
Chernov, I. Yu. [1 ]
机构：
[1] Lomonosov Moscow State Univ, Moscow, Russia
来源：
MICROBIOLOGY | 2016年 / 85卷 / 06期
基金：
俄罗斯基础研究基金会; 俄罗斯科学基金会;
关键词：
yeasts; plant hormones; IAA; auxin; HPLC-MS/MS; PLANT-GROWTH; ACID;
D O I：
10.1134/S0026261716060187
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
High performance liquid chromatography coupled with tandem mass spectrometry (HPLC-MS/MS) was applied to the comprehensive analysis of the ability of yeast strains to synthesize a plant hormone indole-3-acetic acid (IAA). A total of 124 strains (37 species) of yeasts isolated from various regions and substrates were studied. Testing of IAA production showed that 92% strains were capable of IAA synthesis. The results indicated that, in general, ascomycetous yeasts were more active auxin producers than basidiomycetous ones. Geographically, strains from tropical regions were the most active IAA producers. Analysis of the substrate variability of the strains showed higher auxin production (on average) by the yeasts isolated from plants compared to the soil isolates, indicating a specific regulatory role of the plant yeast population.
引用
收藏
页码：727 / 736
页数：10
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