Enzyme-Free and Amplified Fluorescence DNA Detection Using Bimolecular Beacons

被引：129

作者：

Huang, Jiahao ^{[1
]}

Su, Xuefen ^{[2
]}

Li, Zhigang ^{[1
]}

机构：

[1] Hong Kong Univ Sci & Technol, Dept Mech Engn, Kowloon, Hong Kong, Peoples R China

[2] Chinese Univ Hong Kong, Prince Wales Hosp, Fac Med, Sch Publ Hlth & Primary Care, Shatin, Hong Kong, Peoples R China

来源：

ANALYTICAL CHEMISTRY | 2012年 / 84卷 / 14期

关键词：

SEQUENCE-SPECIFIC DETECTION; MOLECULAR BEACONS; SIGNAL AMPLIFICATION; LABEL-FREE; HYBRIDIZATION; MODEL; PROBE; NANOPARTICLES; SENSITIVITY; BIOSENSOR;

D O I：

10.1021/ac3004727

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

In this work, we propose a simple and enzyme-free strategy for sensitive and selective DNA detection by using two different types of molecular beacons (MBs), MB1 and MB2. In this method, the target DNA binds with and restores the fluorescence of MB1 first. Then, MB2 hybridizes with MB1 and free the target, which is used to trigger another reaction cycle. The cycling use of the target and the employment of bi-MBs amplify the fluorescence intensity for sensitive DNA detection. The detection limit of MB 2 this method was obtained as 10 pM, which is about 2 orders of magnitude sensitive than the conventional MB-based approaches.

引用

页码：5939 / 5943

页数：5

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